지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Viral protein 2 (VP2), which is the structural protein of parvovirus, can produce virus-like particles (VLPs) by a self-assembly process in vitro, making VLPs attractive vaccine candidates. VP2 of canine parvovirus (CPV) is responsible for neutralizing antibodies in immunized animals. In this study, VP2 protein of canine parvovirus-2c was expressed using a baculovirus expression system and assembled into parvovirus-like particles in insect cells. The results show that VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and a high hemagglutination (HA) titer (1:27). The recombinant 6-His-tagged VP2 protein with a molecular mass of about 65 kDa was detected by anti-His antibody and anti-PPV serum. This study provides a foundation for the application of VP2 protein in the clinical diagnosis of CPV and in the vaccination against CPV.
목차
등록된 정보가 없습니다.
참고문헌 (9)
참고문헌 신청
Buonavoglia C / 1983 / Dog response to plaque variant of canine parvovirus / Zentralbl Veterinarmed B 30(7):526~531
Carderon MG / 2009 / Molecular characterization of canine parvovirus strains in Argentina : Detection of the pathogenic variant CPV2c in vaccinated dogs / J Virol Methods 159(2):141~145
Decaro N / 2012 / Canine parvovirus-a review of epidemiological and diagnostic aspects, with emphasis on type 2c / Vet Microbiol 155(1):1~12
Decaro N / 2008 / Evidence for immunization failure in vaccinated adult dogs infected with canine parvovirus type 2c / New Microbiol 31(1):125~130
Feng H / 2014 / Canine parvovirus VP2 protein expressed in silkworm pupae self-assembles into virus-like particles with high immunogenicity / PLoS One 9(1):e79575
Carderon MG / 2009 / Molecular characterization of canine parvovirus strains in Argentina : Detection of the pathogenic variant CPV2c in vaccinated dogs / J Virol Methods 159(2):141~145
Decaro N / 2012 / Canine parvovirus-a review of epidemiological and diagnostic aspects, with emphasis on type 2c / Vet Microbiol 155(1):1~12
Decaro N / 2008 / Evidence for immunization failure in vaccinated adult dogs infected with canine parvovirus type 2c / New Microbiol 31(1):125~130
Feng H / 2014 / Canine parvovirus VP2 protein expressed in silkworm pupae self-assembles into virus-like particles with high immunogenicity / PLoS One 9(1):e79575
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