Implementation and Validation of a Gas Chromatography-Mass Spectrometry Method for Pristanic Acid and Phytanic Acid Quantification in Plasma Specimens

Ahram Yi; Jun Hyung Lee; Gahyun Yoo; Hye Jin Lim; Euna Park; Jungsun Han; Geun Young Kim; Sung-Eun Cho; Sang Gon Lee; Eun Hee Lee

doi:10.15263/jlmqa.2021.43.3.162

추천

검색

자료유형: 학술저널

저자정보: Ahram Yi (Green Cross Laboratories (GC Labs)) Jun Hyung Lee (Green Cross Laboratories (GC Labs)) Gahyun Yoo (Green Cross Laboratories (GC Labs)) Hye Jin Lim (Green Cross Laboratories (GC Labs)) Euna Park (Green Cross Laboratories (GC Labs)) Jungsun Han (Green Cross Laboratories (GC Labs)) Geun Young Kim (Green Cross Laboratories (GC Labs)) Sung-Eun Cho (Green Cross Laboratories (GC Labs)) Sang Gon Lee (Green Cross Laboratories (GC Labs)) Eun Hee Lee (Green Cross Laboratories (GC Labs))

저널정보: 대한임상검사정도관리협회 Journal of Laboratory Medicine And Quality Assurance Laboratory Medicine and Quality Assurance 제43권 제3호

발행연도: 2021.9

수록면: 162 - 165 (4page)

DOI: 10.15263/jlmqa.2021.43.3.162

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

We validated a gas chromatography-mass spectrometry (GC-MS) method for quantifying pristanic acid (PrA) and phytanic acid (PhA) in plasma specimens. We developed a GC-MS method based on the analysis of 20 μM 2H3-pristanic acid (PrA-IS) and 80 μM 2H3-phytanic acid (PhA-IS) as internal standards. The GC-MS was fitted with a 30 m×0.25 mm×0.25 μm HP-5MS (Agilent, USA) column. The mass spectrometer was operated through the transitions from the precursor to the product ions (m/z [mass-to-charge ratio] 355, 369, 358, and 372 for PrA, PhA, PrA-IS, and PhA-IS, respectively). The retention times of PrA, PhA, PrA-IS, and PhA-IS were 19.33, 20.39, 19.31, and 20.38 minutes in a 26.83-minute analysis, respectively. Linearity, recovery, precision, and carryover were evaluated to validate the method. The GC-MS method yielded a linear response from 0.032 to 9.951 μmol/L for PrA (R 2=0.9999) and from 0.127 to 39.432 μmol/L for PhA (R 2=0.9998). The limits of quantification by the methods were 0.032 μmol/L for PrA and 0.127 μmol/L for PhA. The recovery of PrA and PhA GC-MS method measurements were within ±10% when evaluated with external quality assessment materials. The within-batch and total coefficients of variation were all below 6% for both PrA and PhA test results. Twenty-interday imprecision (%) were all below 5% for both PrA and PhA test results. Carry-over was found to be 0.001% for PrA and ?0.008% for PhA. The GC-MS PrA and PhA assay showed adequate recovery, precision, sensitivity, and linearity. Hence, it is suitable for routine clinical work.

#Pristanic acid #Phytanic acid #Gas chromatography-mass spectrometry #Method validation

참고문헌 (9)

참고문헌 신청

Vreken P / 1998 / Rapid stable isotope dilution analysis of very-long-chain fatty acids, pristanic acid and phytanic acid using gas chromatography-electron impact mass spectrometry / J Chromatogr B Biomed Sci Appl 713:281~287

Clinical and Laboratory Standards Institute / 2014 / Evaluation of precision of quantitative measurement procedures

Whang DH / 2021 / Laboratory medicine / PanMun Education :73~76

U.S. Department of Health and Human Services / 2018 / Bioanalytical method validation: guidance for industry

Clinical and Laboratory Standards Institute / 2003 / Evaluation of the linearity of quantitative measurement procedures: a statistical approach

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!