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논문 기본 정보

자료유형
학술저널
저자정보
Nabi Jomehzadeh (Department of Microbiology School of Medicine Abadan University of Medical Sciences) Khadijeh Ahmadi (Department of Microbiology School of Medicine Abadan University of Medical Sciences) Zahra Rahmani (Abadan University of Medical Sciences)
저널정보
질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Perspectives 제12권 제6호
발행연도
2021.12
수록면
390 - 395 (6page)
DOI
10.24171/j.phrp.2021.0272

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Objectives: This study was undertaken to evaluate AmpC β-lactamase-producing Escherichia coli urine isolates and to characterize the frequency of plasmid-mediated AmpC (pAmpC)-encoding genes.Methods: Antimicrobial susceptibility tests were performed using the disk diffusion technique. AmpC β-lactamase production was assessed with a phenotypic inhibitor-based method. The presence of 6 pAmpC-encoding cluster genes was detected by multiplex polymerase chain reaction (PCR).Results: The proportion of antibiotic resistance of E. coli isolates ranged from 7.4% to 90.5%, and more than half (51.6%) of the total isolates were multidrug-resistant (MDR). Among the 95 E. coli isolates, 60 (63.2%) were found to be cefoxitin-resistant, but only 14 (14.7%) isolates were confirmed as AmpC β-lactamase-producers. In the PCR assay, pAmpC-encoding genes were found in 15 (15.8%) isolates, and blaDHA was the most prevalent type. However, blaFOX, blaMOX, and blaACC genes were not detected in the isolates.Conclusion: Our findings contributed valuable information concerning antibiotic resistance, confirmatory phenotypic testing for AmpC production, and pAmpC β-lactamase gene content in E. coli isolates in southwestern Iran. The level of MDR recorded in AmpC-producing strains of this study was worrying; therefore, implementing strong infection control approaches to reduce the MDR burden is recommended.

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