Implantation of Bone Marrow Mesenchymal Stem Cells into Small Intestinal Submucosa Improves Bile Duct Injury in Rabbits

Li Ying; Wang Piao; Hu Xiao-dong; Zeng Jing-da; Fang Cheng; Gan Yu; Peng Fang-yi; Yang Xiao-li; Luo De; Li Bo; Su Song

doi:10.1007/s13770-021-00351-2

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자료유형: 학술저널

저자정보: Li Ying (The Affiliated Hospital of Southwest Medical University) Wang Piao (The Affiliated Hospital of Southwest Medical University) Hu Xiao-dong (The Affiliated Hospital of Southwest Medical University) Zeng Jing-da (The Affiliated Hospital of Southwest Medical University) Fang Cheng (The Affiliated Hospital of Southwest Medical University) Gan Yu (The Affiliated Hospital of Southwest Medical University) Peng Fang-yi (The Affiliated Hospital of Southwest Medical University) Yang Xiao-li (The Affiliated Hospital of Southwest Medical University) Luo De (The Affiliated Hospital of Southwest Medical University) Li Bo (The Affiliated Hospital of Southwest Medical University) Su Song (The Affiliated Hospital of Southwest Medical University)

저널정보: 한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제18권 제5호

발행연도: 2021.10

수록면: 887 - 893 (7page)

DOI: 10.1007/s13770-021-00351-2

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BACKGROUND: Bile duct injury (BDI), which may occur during cholecystectomy procedures and living-donor liver transplantation, leads to life-altering complications and significantly increased mortality and morbidity. Tissue engineering, as an emerging method, has shown great potential to treat BDI. Here, we aimed to explore the application of small intestinal submucosa (SIS) matrix composites with bone marrow mesenchymal stem cells (BMSCs) to treat BDI in a rabbit model. METHODS: Rabbit-derived BMSCs were used as seed cells. Porcine SIS was used as the support material. Five centimetres of the common bile duct was dissected, and 1/3?1/2 of the anterior wall diameter was transversely incised to construct the rabbit BDI model. Then, SIS materials without/with BMSCs were inserted into the common bile duct of the BDI rabbits. After 1, 2, 4, and 8 weeks of implantation, the common bile duct was removed. Haematoxylin and eosin (HE) staining was used to assess pathological alterations in the common bile duct, while immunohistochemical staining and western blotting were used to detect expression of the epithelial cell markers CK19 and E-cadherin. Scanning electron microscopy was used to evaluate BMSC growth. RESULTS: Compared with BMSCs alone, SIS-attached BMSCs had increased growth. HE staining showed that the injured bile duct healed well and that the complex gradually degraded as the time from implantation increased. Immunohistochemical staining and western blotting showed that compared with the control group, the in vivo complex group had significantly elevated expression levels of CK19 and E-cadherin. CONCLUSION: BMSC implantation into SIS could improve BDI in rabbits, which might have clinical value for BDI treatment.

#Bone marrow mesenchymal stem cells #Small intestinal submucosa #Bile duct injury #Epithelial cells

참고문헌 (15)

참고문헌 신청

Lau WY / 2010 / Management of bile duct injury after laparoscopic cholecystectomy : a review / ANZ J Surg 80:75~81

Buddingh KT / 2011 / Lower rate of major bile duct injury and increased intraoperative management of common bile duct stones after implementation of routine intraoperative cholangiography / J Am Coll Surg 213:267~274

Pekolj J / 2013 / Intraoperative management and repair of bile duct injuries sustained during 10, 123 laparoscopic cholecystectomies in a high-volume referral center / J Am Coll Surg 216:894~901

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