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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.12
- 수록면
- 1,140 - 1,148 (9page)
- DOI
- 10.9799/ksfan.2017.30.6.1140
이용수
초록· 키워드
오류제보하기
For the purpose of developing new immunomodulatory agents from broccoli, ethanol extract (BCEE), hot water extract (BCHW), and crude polysaccharide (BCCP) were isolated from broccoli, and their immunomodulatory activities and chemical properties were examined. In the in vitro cytotoxicity analysis, BCHW and BCCP did not affect the growth of tumor cells and normal cells. Murine peritoneal macrophages stimulated with BCCP showed higher production of IL-6, IL-12, and TNF- α cytokines than those stimulated with BCHW. Also, BCHW and BCCP did not show proliferation of splenic lymphocytes.
In the in vitro assay for intestinal immunomodulatory activities, only BCCP enhanced GM-CSF secretion and the bone marrow cell-proliferating activity via cells in Peyer’s patches at 1,000 μg/mL. Also, BCHW mainly contained 33.7% neutral sugars, such as arabinose, glucose, and galactose, and 30.7% uronic acid, and BCCP consisted of 42.6% neutral sugars, including arabinose, galactose, and glucose, and 50.5% uronic acid. The above results lead us to conclude that crude polysaccharide (BCCP) isolated from broccoli causes considerably high cytokine production in peritoneal macrophages and bone marrow cell proliferation, and the polysaccharide extraction process is indispensable for separation of new immunomodulatory agents from broccoli.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Abbas AK / 2014 / Cellular and Molecular Immunology / Elsevier Health Sciences :2~5
Blumenkrantz N / 1973 / New method for quantitative determination of uronic acids / Anal Biochem 54:484~489
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Cebra JJ / 1994 / Handbook of Mucosal Immunology / Academic Press :151~158
David CW / 2003 / Cell proliferation, apoptosis, and B-and T-lymphocytes in Peyer's patches of the ileum, in thymus and in lymph nodes of preterm calves, and in full-term calves at birth and on day 5 of life / J Dairy Sci 86:3321~3329
Blumenkrantz N / 1973 / New method for quantitative determination of uronic acids / Anal Biochem 54:484~489
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Cebra JJ / 1994 / Handbook of Mucosal Immunology / Academic Press :151~158
David CW / 2003 / Cell proliferation, apoptosis, and B-and T-lymphocytes in Peyer's patches of the ileum, in thymus and in lymph nodes of preterm calves, and in full-term calves at birth and on day 5 of life / J Dairy Sci 86:3321~3329
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