지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 246 - 254 (9page)
- DOI
- 10.5010/JPB.2021.48.4.246
이용수
초록· 키워드
오류제보하기
Environmental stresses are estimated to have reduced global crop yields of wheat by 5.5%. However, traditional approaches for the transfer of resistance to these stresses in wheat plants have yielded limited results. In this regard, genetic transformation has undoubtedly opened up new avenues to overcome crop losses due to various abiotic stresses. Particle bombardment has been successfully em- ployed for obtaining transgenic wheat. However, most of these procedures employ immature embryos, which are not available throughout the year. Therefore, the present investi- gation utilized mature seeds as the starting material and used the calli raised from three Moroccan durum wheat varieties as the target tissue for genetic transformation by the biolistic approach. The pANIC-5E plasmid containing the SINA gene for drought and salinity tolerance was used for genetic trans- formation. To enhance the regeneration capacity and trans- formation efficiency of the tested genotypes, the study compared the effect of copper supplementation in the induc- tion medium (up to 5 μM) with the standard MS medium. The results show that the genotypes displayed different sensitivities to CuSO4, indicating that the transformation efficiency was highly genotype-dependent. The integration of transgenes in the T0 transformants was demonstrated by polymerase chain reaction (PCR) analysis of the obtained resistant plantlets with primers specific to the SINA gene. Among the three genotypes studied, ‘Isly’ showed the highest efficiency of 9.75%, followed by ‘Amria’ with 1.25% and ‘Chaoui’ with 1%.
목차
등록된 정보가 없습니다.
참고문헌 (52)
참고문헌 신청
Allison H / 2002 / Efficiency agrobacterium-mediated wheat transformation method
Cai L / 2014 / Optimization of a biolistic transformation system for transfer of antifreeze gene KN2 and the bar herbicide resistance gene in common wheat / Genet Mol Res 13(2):3474~3485
Cho MJ / 1998 / Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism / Plant Sci 138(2):229~244
Dahleen LS / 1995 / Improved plant regeneration from barley cultures by increased copper levels / Plant Cell Tiss Organ Cult 43:267~269
Ekom D / 2014 / Immature Embryo-Derived of Two Bread Wheat (Triticum aestivum L.) Varieties Transformation Using Particle Bombardment Method / Annu. Res. Rev. Biol 4
Cai L / 2014 / Optimization of a biolistic transformation system for transfer of antifreeze gene KN2 and the bar herbicide resistance gene in common wheat / Genet Mol Res 13(2):3474~3485
Cho MJ / 1998 / Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism / Plant Sci 138(2):229~244
Dahleen LS / 1995 / Improved plant regeneration from barley cultures by increased copper levels / Plant Cell Tiss Organ Cult 43:267~269
Ekom D / 2014 / Immature Embryo-Derived of Two Bread Wheat (Triticum aestivum L.) Varieties Transformation Using Particle Bombardment Method / Annu. Res. Rev. Biol 4
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