YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Li Weijian; Chen Gaohuang; Feng Zhenyu; Zhu Baoyi; Zhou Lilin; Zhang Yuying; Mai Junyan; Jiang Chonghe; Zeng Jianwen

doi:10.1007/s13258-021-01175-z

추천

검색

자료유형: 학술저널

저자정보: Li Weijian (The Sixth Affiliated Hospital of Guangzhou Medical University) Chen Gaohuang (The Sixth Affiliated Hospital of Guangzhou Medical University) Feng Zhenyu (The Sixth Affiliated Hospital of Guangzhou Medical University) Zhu Baoyi (The Sixth Affiliated Hospital of Guangzhou Medical University) Zhou Lilin (The Sixth Affiliated Hospital of Guangzhou Medical University) Zhang Yuying (The Sixth Affiliated Hospital of Guangzhou Medical University) Mai Junyan (The Sixth Affiliated Hospital of Guangzhou Medical University) Jiang Chonghe (The Sixth Affiliated Hospital of Guangzhou Medical University) Zeng Jianwen (The Sixth Affiliated Hospital of Guangzhou Medical University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.43 No.12

발행연도: 2021.12

수록면: 1,413 - 1,421 (9page)

DOI: 10.1007/s13258-021-01175-z

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Diferentially expressed mRNAs were identifed by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verifed that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

#YTHDF1 #TRIM44 #Prostate cancer cells #Migration #Invasion

참고문헌 (33)

참고문헌 신청

Chen L / 2021 / Inhibition of SPATS2 suppresses proliferation and invasion of hepatocellular carcinoma cells through TRIM44-STAT3 signaling pathway / J Cancer 12:89~98

Chen P / 2021 / Targeting YTHDF1 efectively re-sensitizes cisplatin-resistant colon cancer cells by modulating GLS-mediated glutamine metabolism / Mol Ther Oncolytics 20:228~239

Chen XY / 2021 / The m(6)A reader YTHDF1 facilitates the tumorigenesis and metastasis of gastric cancer via USP14 translation in an m(6)A-dependent manner / Front Cell Dev Biol 9:647702

Haberkorn U / 2016 / New strategies in prostate cancer : prostate-specifc membrane antigen(PSMA)ligands for diagnosis and therapy / Clin Cancer Res 22:9~15

Huang H / 2020 / M(6)A modifcation in coding and non-coding RNAs : roles and therapeutic implications in cancer / Cancer Cell 37:270~288

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!