Osteopontin accelerates the development and metastasis of bladder cancer via activating JAK1/STAT1 pathwayOsteopontin accelerates the development and metastasis of bladder cancer via activating JAK1/STAT1 pathway

Na Zhang; Fei Li; Juanyu Gao; Shibao Zhang; Qihong Wang

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자료유형: 학술저널

저자정보: Na Zhang (Ji’nan Central Hospital Affiliated to Shandong University) Fei Li (Ji’nan Central Hospital Affiliated to Shandong University) Juanyu Gao (Ji’nan Central Hospital Affiliated to Shandong University) Shibao Zhang (Ji’nan Central Hospital Affiliated to Shandong University) Qihong Wang (Ji’nan Central Hospital Affiliated to Shandong University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.42 No.4

발행연도: 2020.1

수록면: 467 - 475 (9page)

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Background Bladder cancer is the 10th common cancer worldwide. Osteopontin has been found to enhance cell proliferation, metastasis and invasion in various human tumors. Objective To investigate the roles of osteopontin in bladder cancer. Methods The RNA interference and overexpression of osteopontin were performed in bladder cancer cell lines (T24 and SCaBER). Cell proliferation and apoptosis were measured using CCK-8 assay and flow cytometry, respectively. Cell invasion was determined using transwell assay. Results Osteopontin was highly expressed in bladder cancer tissues in comparison with the adjacent normal tissues. Its high expression significantly correlated with high histologic grade, high TNM stage (III and IV) and poor prognosis. For T24 cells with osteopontin interference and SCaBER cells with osteopontin overexpression, cell proliferation was significantly inhibited (3.58-fold vs. 5.62-fold) and enhanced (7.81-fold vs. 5.29-fold), respectively. The apoptosis portion of T24 cells significantly increased from 4.48 to 10.75%, and that of SCaBER cells significantly declined from 7.33 to 4.01%. The invaded T24 and SCaBER cells significantly decreased to 52.0% and increased to 2.0-fold, respectively. Osteopontin overexpression enhanced the expression (1.54-fold and 2.39-fold; 2.33-fold and 2.05-fold) and activation (1.80-fold and 1.96-fold; 2.00-fold and 2.59-fold) of JAK1 and STAT1 in two cell lines of bladder cancer. Conclusion Osteopontin might enhance proliferation, inhibit apoptosis and accelerate invasion and thus promote the development and metastasis of bladder cancer, and osteopontin’s functions might be mediated by activating JAK1/STAT1 signaling pathway.

#Bladder cancer · Invasion · JAK1/STAT1 pathway · Osteopontin · Proliferation

참고문헌 (24)

참고문헌 신청

Behera R / 2010 / Activation of JAK2/STAT3 signaling by osteopontin promotes tumor growth in human breast cancer cells / Carcinogenesis 31:192~200

Bray F / 2018 / Global cancer statistics 2018 : GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries / CA Cancer J Clin 68:394~424

Chen X / 2017 / Heterogeneous nuclear ribonucleoprotein K is associated with poor prognosis and regulates proliferation and apoptosis in bladder cancer / J Cell Mol Med 21:1266~1279

Chen Z / 2018 / HBO1 promotes cell proliferation in bladder cancer via activation of Wnt/beta-catenin signaling / Mol Carcinogen 57:12~21

Forootan SS / 2006 / Prognostic significance of osteopontin expression in human prostate cancer / Int J Cancer 118:2255~2261

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