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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 1,157 - 1,165 (9page)
- DOI
- 10.1007/s13258-021-01117-9
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초록· 키워드
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Background Gliomas are common malignant tumors in the nervous system, known for poor prognosis and low survival rate.
Objective This study aims to explore functions of miR-137 in glioma progression and identify messenger RNAs (mRNA) regulated by miR-137, which provides new ideas for further exploration of glioma therapeutic targets.
Methods Gene expression data were downloaded from the Cancer Genome Atlas database, and abnormally expressed miRNAs and mRNAs in glioma were analyzed. The expression of genes in 20 pairs of clinical tissue samples and glioma cell lines were detected through qRT-PCR, and the expression of proteins was detected through Western blot. Changes in cell proliferative level after transfection were detected via CCK8 assay, and changes in cell migratory and invasive abilities were detected by Transwell assay. Besides, dual-luciferase reporter assay was employed to testify binding relationship between two genes.
Results Our study found that miR-137 was signifcantly and lowly expressed in glioma tissue and cell lines, and the prognoses of glioma patients with highly expressed miR-137 were more optimistic. Overexpressed miR-137 could remarkably inhibit proliferative, invasive and migratory abilities of glioma cells U87, while transfection of miR-137 inhibitor presented an opposite efect. Additionally, EZH2 was a direct target of miR-137 and overexpressed EZH2 efectively reversed the efect of miR-137 on glioma proliferation and migration.
Conclusions Our study found that miR-137 could suppress the proliferation, invasion and migration of glioma cells through regulating the expression of EZH2. So far, we have found a novel regulatory pair that infuences glioma progression, providing a basis for further development of new therapeutic strategies.
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참고문헌 (25)
참고문헌 신청
Bi WP / 2018 / miR-137 suppresses proliferation, migration and invasion of colon cancer cell lines by targeting TCF4 / Oncol Lett 15:8744~8748
Chen R / 2017 / miR-137 inhibits the proliferation of human non-small cell lung cancer cells by targeting SRC3 / Oncol Lett 13:3905~3911
Di Leva G / 2014 / MicroRNAs in cancer / Annu Rev Pathol 9:287~314
Ding F / 2018 / MiR-137functions as a tumor suppressor in pancreatic cancer by targeting MRGBP / J Cell Biochem 119:4799~4807
Esquela-Kerscher A / 2006 / Oncomirs : microRNAs with a role in cancer / Nat Rev Cancer 6:259~269
Chen R / 2017 / miR-137 inhibits the proliferation of human non-small cell lung cancer cells by targeting SRC3 / Oncol Lett 13:3905~3911
Di Leva G / 2014 / MicroRNAs in cancer / Annu Rev Pathol 9:287~314
Ding F / 2018 / MiR-137functions as a tumor suppressor in pancreatic cancer by targeting MRGBP / J Cell Biochem 119:4799~4807
Esquela-Kerscher A / 2006 / Oncomirs : microRNAs with a role in cancer / Nat Rev Cancer 6:259~269
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