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논문 기본 정보

자료유형
학술저널
저자정보
Huang Zhen (Key Laboratory of Animal Nutrition of Jiangxi Province Nutritional Feed Development Engineering Res) Ni Guorong (College of Land Resources and Environment Jiangxi Agriculture University Nanchang P.R. China) Wang Fei (College of Bioscience and Bioengineering Jiangxi Agricultural University Nanchang 330045 P.R. China) Zhao Xiaoyan (College of Bioscience and Bioengineering Jiangxi Agricultural University Nanchang 330045 P.R. China) Yunda Chen (College of Bioscience and Bioengineering Jiangxi Agricultural University Nanchang 330045 P.R. China) Zhang Lixia (College of Bioscience and Bioengineering Jiangxi Agricultural University Nanchang 330045 P.R. China) Qu Mingren (Key Laboratory of Animal Nutrition of Jiangxi Province Nutritional Feed Development Engineering Res)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제4호
발행연도
2022.4
수록면
484 - 492 (9page)
DOI
10.4014/jmb.2111.11017

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Lichenase is an enzyme mainly implicated in the degradation of polysaccharides in the cell walls of grains. Emerging evidence shows that a highly efficient expression of a thermostable recombinant lichenase holds considerable promise for application in the beer-brewing and animal feed industries. Herein, we cloned a lichenase gene (CelA203) from Bacillus subtilis B110 and expressed it in E. coli. This gene contains an ORF of 729 bp, encoding a protein with 242 amino acids and a calculated molecular mass of 27.3 kDa. According to the zymogram results, purified CelA203 existed in two forms, a monomer, and a tetramer, but only the tetramer had potent enzymatic activity. CelA203 remained stable over a broad pH and temperature range and retained 40% activity at 70°C for 1 h. The Km and Vmax of CelA203 towards barley β-glucan and lichenan were 3.98 mg/ml, 1017.17 U/mg, and 2.78 mg/ml, 198.24 U/mg, respectively. Furthermore, trisaccharide and tetrasaccharide were the main products obtained from CelA203-mediated hydrolysis of deactivated oat bran. These findings demonstrate a promising role for CelA203 in the production of oligosaccharides in animal feed and brewing industries.

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