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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제7호
- 발행연도
- 2017.7
- 수록면
- 1,257 - 1,265 (9page)
- DOI
- 10.4014/jmb.1704.04033
이용수
초록· 키워드
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The present study was carried out to investigate the antioxidative and neuroprotective effects of sea buckthorn (Hippophae rhamnoides L.) leaves (SBL) harvested at different times. Reversedphase high-performance liquid chromatography analysis revealed five major phenolic compounds: ellagic acid, gallic acid, isorhamnetin, kaempferol, and quercetin. SBL harvested in August had the highest total phenolic and flavonoid contents and antioxidant capacity.
Treatment of neuronal PC-12 cells with the ethyl acetate fraction of SBL harvested in August increased their viability and membrane integrity and reduced intracellular oxidative stress in a dose-dependent manner. The relative populations of both early and late apoptotic PC-12 cells were decreased by treatment with the SBL ethyl acetate fraction, based on flow cytometry analysis using annexin V-FITC/PI staining. These findings suggest that SBL can serve as a good source of antioxidants and medicinal agents that attenuate oxidative stress.
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참고문헌 (39)
참고문헌 신청
Rousi A / 1971 / The genus Hippophaë L. A taxonomic study / Ann. Bot. Fennici 8:177~227
Zeb A. / 2004 / Important therapeutic uses of sea buckthorn (Hippophae): a review / J. Biol. Sci 4:687~693
Guliyev VB / 2004 / Hippophae rhamnoides L.:chromatographic methods to determine chemical composition, use in traditional medicine and pharmacological effects / J. Chromatogr. B 812:291~307
Eastwood MA. / 1999 / Interaction of dietary antioxidants in vivo: how fruit and vegetables prevent disease? / Q. J. Med 92:527~530
Nimse SB / 2015 / Free radicals, natural antioxidants, and their reaction mechanisms / RSC Adv 5:27986~28006
Zeb A. / 2004 / Important therapeutic uses of sea buckthorn (Hippophae): a review / J. Biol. Sci 4:687~693
Guliyev VB / 2004 / Hippophae rhamnoides L.:chromatographic methods to determine chemical composition, use in traditional medicine and pharmacological effects / J. Chromatogr. B 812:291~307
Eastwood MA. / 1999 / Interaction of dietary antioxidants in vivo: how fruit and vegetables prevent disease? / Q. J. Med 92:527~530
Nimse SB / 2015 / Free radicals, natural antioxidants, and their reaction mechanisms / RSC Adv 5:27986~28006
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