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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제1호
- 발행연도
- 2018.1
- 수록면
- 40 - 49 (10page)
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We evaluated the antioxidant activity and neuronal cell-protective effect of fucoidan extract from Ecklonia cava (FEC) on hydrogen peroxide (H2O2)-induced cytotoxicity in PC-12 and MCIXC cells to assess its protective effect against oxidative stress. Antioxidant activities were examined using the ABTS radical scavenging activity and malondialdehyde-inhibitory effect, and the results showed that FEC had significant antioxidant activity. Intracellular ROS contents and neuronal cell viability were investigated using the DCF-DA assay and MTT reduction assay. FEC also showed remarkable neuronal cell-protective effect compared with vitamin C as a positive control for both H2O2-treated PC-12 and MC-IXC cells. Based on the neuronal cellprotective effects, mitochondrial function was analyzed in PC-12 cells, and FEC significantly restored mitochondrial damage by increasing the mitochondrial membrane potential (Δψm) and ATP levels and regulating mitochondrial-mediated proteins (p-AMPK and BAX). Finally, the inhibitory effects against acetylcholinesterase (AChE), which is a critical hydrolyzing enzyme of the neurotransmitter acetylcholine in the cholinergic system, were investigated (IC50 value = 1.3 mg/ml) and showed a mixed (competitive and noncompetitive) pattern of inhibition. Our findings suggest that FEC may be used as a potential material for alleviating oxidative stress-induced neuronal damage by regulating mitochondrial function and AChE inhibition.
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참고문헌 (41)
참고문헌 신청
Wang J / 2010 / Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica / Int. J. Biol. Macromol. 46:6~12
Mancuso M / 2006 / Mitochondrial dysfunction, oxidative stress and neurodegeneration / J. Alzheimers Dis. 10:59~73
Gao J / 2017 / Icariside II, a novel phosphodiesterase 5 inhibitor, protects against $H_2O_2$ -induced PC12 cells death by inhibiting mitochondria-mediated autophagy / J. Cell. Mol. Med. 21:375~386
Elmann A / 2009 / Protective effects of the essential oil of Salvia fruticosa and its constituents on astrocytic susceptibility to hydrogen peroxide-induced cell death / J. Agric. Food Chem. 57:6636~6641
Rigoulet M / 2011 / Mitochondrial ROS generation and its regulation: mechanisms involved in $H_2O_2$ signaling / Antioxid. Redox. Signal. 14:459~468
Mancuso M / 2006 / Mitochondrial dysfunction, oxidative stress and neurodegeneration / J. Alzheimers Dis. 10:59~73
Gao J / 2017 / Icariside II, a novel phosphodiesterase 5 inhibitor, protects against
Elmann A / 2009 / Protective effects of the essential oil of Salvia fruticosa and its constituents on astrocytic susceptibility to hydrogen peroxide-induced cell death / J. Agric. Food Chem. 57:6636~6641
Rigoulet M / 2011 / Mitochondrial ROS generation and its regulation: mechanisms involved in
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