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논문 기본 정보

자료유형
학술저널
저자정보
Park Young Shik (Department of Biotechnology and Biomarketing The University of Suwon Hwaseong 18323 Republic of Kor) Lee Jae Yeon (Department of Biotechnology and Biomarketing The University of Suwon Hwaseong 18323 Republic of Kor) Hwang Kyo Yeol (Department of Biotechnology and Biomarketing The University of Suwon Hwaseong 18323 Republic of Kor) 김근 (수원대학교)
저널정보
한국미생물생명공학회 한국미생물·생명공학회지 한국미생물·생명공학회지 제49권 제4호
발행연도
2021.12
수록면
566 - 575 (10page)
DOI
10.48022/mbl.2111.11008

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Galactose and soybean meal were selected as the best carbon and nitrogen sources, repectively, for the efficient production of α-glucosidase inhibitor (AGI) and 1-deoxynojirimycin (DNJ) by a newly isolated Bacillus subtilis MORI. The optimal concentrations of the galactose and soybean meal for the production of AGI and DNJ were investigated by response surface methodology. For the production of AGI, the optimal galactose and soybean meal concentrations were 4.3% (w/v) and 3.2% (w/v), respectively, and for DNJ, 4.5% (w/v) and 3.0% (w/v), respectively. The nearly identical optimal concentrations of galactose and soybean meal for the production of both AGI and DNJ indicated a close correlation between the production of AGI and DNJ. The maximum production of AGI (50,880 GIU/ml) and DNJ (824 μg/ml) obtained from the statistically optimized medium in a jar fermenter was 2.33 and 2.38-fold, respectively, higher than those (21,798 GIU/ml and 346 μg/ml, respectively) of the pre-optimized medium. The production of both AGI and DNJ was greatly increased by jar fermentation (AGI of 38,524 GIU/ml and DNJ of 491 μg/ml) compared with flask fermentation.

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