지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.8
- 수록면
- 1 - 16 (16page)
- DOI
- 10.1038/s12276-020-0475-0
이용수
초록· 키워드
오류제보하기
Our study aimed to determine how lncRNA DANCR, miR-320a, and CTNNB1 interact with each other and regulate osteogenic differentiation in osteoporosis. qRT-PCR and western blotting were performed to determine the expression of DANCR, miR-320a, CTNNB1, and the osteoporosis- or Wnt/β-catenin pathway-related markers T-cell factor 1 (TCF-1), runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN). Interactions between CTNNB1, DANCR, and miR-320a were predicted by bioinformatics approaches and validated using a luciferase assay. Osteoblastic phenotypes were evaluated by ALP staining, ALP activity assay and Alizarin Red staining. The bilateral ovariectomy method was used to establish an in vivo osteoporosis model. Bone morphological changes were examined using hematoxylin and eosin (H&E) and Alcian Blue staining. The expression levels of DANCR and miR-320a in BMSCs derived from osteoporosis patients were upregulated, whereas CTNNB1 expression was downregulated compared with that in healthy controls. Importantly, we demonstrated that miR-320a and DANCR acted independently from each other and both inhibited CTNNB1 expression, whereas the inhibitory effect was additive when miR-320a and DANCR were cooverexpressed. Moreover, we found that DANCR overexpression largely abrogated the effect of the miR-320a inhibitor on CTNNB1 expression and the Wnt/β-catenin signaling pathway in BMSCs during osteogenic differentiation. We further con?rmed the results above in BMSCs derived from an osteoporosis animal model. Taken together, our ?ndings revealed that DANCR and miR-320a regulated the Wnt/ β-catenin signaling pathway during osteogenic differentiation in osteoporosis through CTNNB1 inhibition. Our results highlight the potential value of DANCR and miR-320a as promising therapeutic targets for osteoporosis treatment.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2
Experimental and Molecular Medicine
2022 .07
-
MiR-34a and miR-34b/c have distinct effects on the suppression of lung adenocarcinomas
Experimental and Molecular Medicine
2019 .01
-
MiR-34a, miR-21 and miR-23a as potential biomarkers for coronary artery disease: a pilot microarray study and confirmation in a 32 patient cohort
Experimental and Molecular Medicine
2015 .01
-
MiR-214 Regulates the Human Hair Follicle Stem Cell Proliferation and Differentiation by Targeting EZH2 and Wnt/b- Catenin Signaling Way In Vitro
조직공학과 재생의학
2018 .01
-
NMR hydrogen exchange study of miR156:miR156* duplexes
Journal of the Korean Magnetic Resonance Society
2019 .01
-
miR-30c regulates proliferation, apoptosis and differentiation via the Shh signaling pathway in P19 cells
Experimental and Molecular Medicine
2016 .01
-
ANT2 shRNA downregulates miR-19a and miR-96 through the PI3K/Akt pathway and suppresses tumor growth in hepatocellular carcinoma cells
Experimental and Molecular Medicine
2016 .01
-
MicroRNA-375 is a therapeutic target for castration-resistant prostate cancer through the PTPN4/STAT3 axis
Experimental and Molecular Medicine
2022 .08
-
Effects of microRNA-135a on the epithelial–mesenchymal transition, migration and invasion of bladder cancer cells by targeting GSK3β through the Wnt/β-catenin signaling pathway
Experimental and Molecular Medicine
2018 .01
-
miR-19b enhances osteogenic differentiation of mesenchymal stem cells and promotes fracture healing through the WWP1/Smurf2-mediated KLF5/β-catenin signaling pathway
Experimental and Molecular Medicine
2021 .05
-
MiR-155 modulates the inflammatory phenotype of intestinal myofibroblasts by targeting SOCS1 in ulcerative colitis
Experimental and Molecular Medicine
2015 .01
-
miR-638 is a new biomarker for outcome prediction of non-small cell lung cancer patients receiving chemotherapy
Experimental and Molecular Medicine
2015 .01
-
microRNA-25 as a novel modulator of circadian Period2 gene oscillation
Experimental and Molecular Medicine
2020 .09
-
Emodin prevents intima thickness via Wnt4/Dvl-1/β-catenin signaling pathway mediated by miR-126 in balloon-injured carotid artery rats
Experimental and Molecular Medicine
2015 .01
-
MiR-125a-5p ameliorates monocrotalineinduced pulmonary arterial hypertension by targeting the TGF-β1 and IL-6/STAT3 signaling pathways
Experimental and Molecular Medicine
2018 .01
-
K-MIR: 한국어 멀티모달 의도 인식 데이터셋
한국정보과학회 학술발표논문집
2024 .12
-
miR-101-3p/Rap1b signal pathway plays a key role in osteoclast differentiation after treatment with bisphosphonates
BMB Reports
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0