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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 572 - 576 (5page)
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초록· 키워드
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Bisphosphonates are the mainstay of therapy worldwide for osteoporosis. However, bisphosphonates also have limitations. The objective of this study was to determine the role of miR-101-3p/Rap1b signal pathway in osteoclast differentiation after treatment with bisphosphonates. Our results revealed that miR-101-3p was an important regulator in bisphosphonates treated-osteoclasts. When miR-101-3p was down-regulated in bone marrow-derived macrophage-like cells (BMMs), the development of mature osteoclasts was promoted, and vice versa. However, alendronate decreased multinucleated cell number regardless of whether miR-101-3p was knocked down or over-expressed. TRAP activity assay confirmed the above results. Luciferase assay indicated that miR-101-3p was a negative regulator of Rap1b. Western blot analysis revealed that protein expression level of Rap1b in BMMs transfected with OV-miR-101-3p was lower than that in BMMs transfected with an empty vector. Rap1b overexpression increased TRAP-positive multinucleated cells, while Rap1b inhibition decreased the cell numbers. In vivo data showed that miR-101-3p inhibited osteoclast differentiation in ovariectomized mice while overexpressed of Rap1b blocked the differentiation. Taken together, our data demonstrate that miR-101-3p/Rap1b signal pathway plays a key role in osteoclast differentiation after treatment with bisphosphonates.
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참고문헌 (24)
참고문헌 신청
Johnell O / 2006 / An estimate of the worldwide prevalence and disability associated with osteoporotic fractures / Osteoporos Int 17:1726~1733
Ji X / 2016 / MicroRNAs in Osteoclastogenesis and Function : Potential Therapeutic Targets for Osteoporosis / Int J Mol Sci 17:349
Yuen T / 2014 / Bisphosphonates inactivate human EGFRs to exert antitumor actions / Proc Natl Acad Sci U S A 111:17989~17994
Soares AP / 2016 / Bisphosphonates : Pharmacokinetics, bioavailability, mechanisms of action, clinical applications in children, and effects on tooth development / Environ Toxicol Pharmacol 42:212~217
Fordham JB / 2016 / MiR-142-3p is a RANKL-dependent inducer of cell death in osteoclasts / Sci Rep 6:24980
Ji X / 2016 / MicroRNAs in Osteoclastogenesis and Function : Potential Therapeutic Targets for Osteoporosis / Int J Mol Sci 17:349
Yuen T / 2014 / Bisphosphonates inactivate human EGFRs to exert antitumor actions / Proc Natl Acad Sci U S A 111:17989~17994
Soares AP / 2016 / Bisphosphonates : Pharmacokinetics, bioavailability, mechanisms of action, clinical applications in children, and effects on tooth development / Environ Toxicol Pharmacol 42:212~217
Fordham JB / 2016 / MiR-142-3p is a RANKL-dependent inducer of cell death in osteoclasts / Sci Rep 6:24980
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