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논문 기본 정보

자료유형
학술저널
저자정보
Largia Muthiah Joe Virgin (Department of Biotechnology Alagappa University) Pandian Subramani (Department of Biotechnology Alagappa University) Shilpha Jayabalan (Department of Biotechnology Alagappa University) Chitradevi Muniyarajan (Huazhong Agricultural University) Kavikkuil Manickam (Huazhong Agricultural University) Sohn Soo In (국립농업과학원) Ramesh Manikandan (Department of Biotechnology Alagappa University)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제16권 제6호
발행연도
2022.12
수록면
621 - 632 (12page)
DOI
10.1007/s11816-022-00775-9

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Justicia gendarussa Burm. f. is an indispensable medicinal plant owing to its abundance of phytoconstituents and medicinal uses. In the study, we demonstrated the successful in vitro regeneration of J. gendarussa through nodal explants and its genetic and phytochemical analysis. A combination of 2 mg L-1 BA + 0.1 mg L-1 NAA + 15 mg L-1 spermidine was found to be reliable in producing the maximum number of multiple shoots (16.6) and vigorous roots in Murashige and Skoog medium. Rooted shoots have been efciently hardened and then slowly acclimatized to natural conditions. SPAR-based genetic fdelity checking revealed the true-to-type nature of in vitro regenerated plants with little variations by generating 105 bands through amplifcation of 15 primers. Furthermore, the protocol followed for in vitro culture was strengthened by the comparative determination of Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and Antioxidant Potential (AP). Methanolic extracts of in vitro plants showed higher amounts of TPC, TFC, and AP when compared to in vivo plants which might be due to the infuence of plant growth hormones. Thus the in vitro regeneration protocol derived would vitally expedite large scale production of true to type plantlets of J. gendarussa. In addition, an attempt was made for the frst time to devise a suitable protocol for the induction of hairy roots from this plant using two Agrobacterium rhizogenes strains which would lead to enhanced production of important anti-HIV metabolites of the plant such as gendarussin A, B and so on.

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