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자료유형
학술저널
저자정보
Islam Md Imtiazul (Department of Microbiology and Immunology School of Medicine Soonchunhyang University Cheonan Chung) Seo Hoonhee (Department of Microbiology and Immunology School of Medicine Soonchunhyang University Cheonan Chung) Redwan Asma (Department of Microbiology and Immunology School of Medicine Soonchunhyang University Cheonan Chung) Kim Sukyung (Department of Microbiology and Immunology School of Medicine Soonchunhyang University Cheonan Chung) Lee Saebim (Department of Microbiology and Immunology School of Medicine Soonchunhyang University Cheonan Chung) Siddiquee Mashuk (Department of Microbiology and Immunology School of Medicine Soonchunhyang University Cheonan Chung) 송호연 (순천향대학교)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제1호
발행연도
2022.1
수록면
46 - 55 (10page)
DOI
10.4014/jmb.2107.07057

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Clostridioides difficile infection (CDI) is a significant cause of hospital-acquired and antibioticmediated intestinal diseases and is a growing global public health concern. Overuse of antibiotics and their effect on normal intestinal flora has increased the incidence and severity of infections. Thus, the development of new, effective, and safe treatment options is a high priority. Here, we report a new probiotic strain, Bacillus amyloliquefaciens (BA PMC-80), and its in vitro/in vivo anti-C. difficile effect as a prospective novel candidate for replacing conventional antibiotics. BA PMC-80 showed a significant anti-C. difficile effect in coculture assay, and its cell-free supernatant (CFS) also exhibited a considerable anti-C. difficile effect with an 89.06 μg/ml 50% minimal inhibitory concentration (MIC) in broth microdilution assay. The CFS was stable and equally functional under different pHs, heat, and proteinase treatments. It also exhibited a high sensitivity against current antibiotics and no toxicity in subchronic toxicity testing in hamsters. Finally, BA PMC-80 showed a moderate effect in a hamster CDI model with reduced infection severity and delayed death. However, further studies are required to optimize the treatment condition of the hamster CDI model for better efficacy and identify the antimicrobial compound produced by BA PMC-80.

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