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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.2
- 수록면
- 165 - 168 (4page)
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초록· 키워드
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Surface protein internalin (InlA) is a major virulence factor of the food-borne pathogen L. monocytogenes.
It plays an important role in bacteria crossing the host's barrier by specific interaction with the cell adhesionmolecule E-cadherin. Study of this protein will help to find better ways to prevent listeriosis. In this study,a monoclonal antibody against InlA was used to detect InlA. The reaction was label-free and monitoredin real time with an oblique-incidence reflectivity-difference (OI-RD) technique. The kinetic constants konand koff and the equilibrium dissociation constant Kd for this reaction were also obtained. These parametersindicate that the antibody is capable of detecting InlA. Additionally, the results also demonstrate thefeasibility of using OI-RD for proteomics research and bacteria detection.
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등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
J. Mengaud / 1996 / E-cadherin is the receptor for internalin, a surface protein required for entry of L. monocytogenes into epithelial cells / Cell 84:923~932
C. D’Souza-Schorey / 2005 / Disassembling adherens junctions:breaking up is hard to do / Trends Cell Biol 15:19~26
A. K. Bhunia / 1992 / Monoclonal antibody specific for Listeria monocytogenes associated with a 66-kilodalton cell surface antigen / Appl. Environ. Microbiol 58:1924~1929
A. K. Bhunia / 1991 / Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and Listeria innocua / Infect. Immun 59:3176~3184
Yoon-Kyu Lim / 2005 / Development of a sandwich ELISA for the detection of Listeria SPP. Using specific flagella antibodies / Journal of Veterinary Science 6(1):41~46
C. D’Souza-Schorey / 2005 / Disassembling adherens junctions:breaking up is hard to do / Trends Cell Biol 15:19~26
A. K. Bhunia / 1992 / Monoclonal antibody specific for Listeria monocytogenes associated with a 66-kilodalton cell surface antigen / Appl. Environ. Microbiol 58:1924~1929
A. K. Bhunia / 1991 / Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and Listeria innocua / Infect. Immun 59:3176~3184
Yoon-Kyu Lim / 2005 / Development of a sandwich ELISA for the detection of Listeria SPP. Using specific flagella antibodies / Journal of Veterinary Science 6(1):41~46
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