Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging

Chenglong Sun; Shuangshuang Ma; Lili Li; Daijie Wang; Wei Liu; Feng Liu; Lanping Guo; Xiao Wang

Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging

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Type: Academic journal

Author: Chenglong Sun (Qilu University of Technology (Shandong Academy of Sciences)) Shuangshuang Ma (Qilu University of Technology (Shandong Academy of Sciences)) Lili Li (Qilu University of Technology (Shandong Academy of Sciences)) Daijie Wang (Qilu University of Technology (Shandong Academy of Sciences))

Journal: The Korean Society of Ginseng Journal of Ginseng Research Vol.45 No.6 KCI Candidated Journals SCIE

Published: 2021.11

Pages: 726 - 733 (8page)

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Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging

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Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asian countries. Its root and rhizome have long been used for the treatment of cardiovascular and hematological diseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous plant tissues is significant for characterizing the metabolic networks of Panax notoginseng, and this will also provide a highly informative approach to understand the complex molecular changes in the processing of Panax notoginseng.
Methods: Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize the spatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panax notoginseng.
Results: A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine, gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizome and root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during the steaming of Panax notoginseng root were also characterized in this study. And, a series of metabolites such as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng were successfully screened out and imaged.
Conclusion: These spatially-resolved metabolite data not only enhance our understanding of the Panax notoginseng metabolic networks, but also provide direct evidence that a serious of metabolic alterations occurred during the steaming of Panax notoginseng.

#Panax notoginseng #Metabolites #Spatial distribution #Steaming process #MALDI-MS imaging

ABSTRACT
1. Introduction
2. Material and methods
3. Results and discussion
4. Conclusion
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Chan HH / 2011 / Bioactive constituents from the roots of Panax japonicus var. major and development of a LC-MS/MS method for distinguishing between natural and artifactual compounds / J Nat Prod 74:796~802

Fang Liu / 2018 / Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector / Journal of Ginseng Research 42(2):149~157

Yin Xiong / 2019 / Chemical and bioactive comparison of Panax notoginseng root and rhizome in raw and steamed forms / Journal of Ginseng Research 43(3):385~393

Zhu D / 2018 / Pharmacokinetic characteristics of steamed notoginseng by an efficient LC-MS/MS method for simultaneously quantifying twenty-three triterpenoids / J Agric Food Chem 66:8187~8198

Lu YY / 2018 / Comparative study on excretive characterization of main components in herb pair notoginsengsafflower and single herbs by LC⁻MS/MS / Pharmaceutics 10

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