지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.4
- 수록면
- 127 - 134 (8page)
이용수
초록· 키워드
오류제보하기
Background: Rhizospheric fungi play an essential role in the plantesoil ecosystem, affecting plant growth and health. In this study, we evaluated the fungal diversity in the rhizosphere soil of 2-yr-old healthy Panax notoginseng cultivated in Wenshan, China.
Methods: Culture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng.
Results: A total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequenceswas high, accounting for 12.9% and 5.5%, respectively. In total,113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus.
Conclusion: Our results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.
Methods: Culture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng.
Results: A total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequenceswas high, accounting for 12.9% and 5.5%, respectively. In total,113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus.
Conclusion: Our results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (53)
참고문헌 신청
[학술지(정기간행물)] - Bouffaud ML / 2012 / Is diversification history of maize influencing selection of soil bacteria by roots? / Mol Ecol 21 : 195 ~ 206
[학술지(정기간행물)] - Xu L / 2012 / Soil fungal community structure along a soil health gradient in pea fields examined using deep amplicon sequencing / Soil Boil Biochem 46 : 26 ~ 32
[학술지(정기간행물)] - Mendes R / 2013 / The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms / FEMS Microbiol Rev 37 : 634 ~ 663
[학술지(정기간행물)] - Singh AK / 2014 / Rhizospheric fungal community structure of a Bt brinjal and a near isogenic variety / J Appl Microbiol 117 : 750 ~ 765
[학술지(정기간행물)] - Kristin A / 2013 / The root microbiotada fingerprint in the soil? / Plant Soil 370 : 671 ~ 686
[학술지(정기간행물)] - Xu L / 2012 / Soil fungal community structure along a soil health gradient in pea fields examined using deep amplicon sequencing / Soil Boil Biochem 46 : 26 ~ 32
[학술지(정기간행물)] - Mendes R / 2013 / The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms / FEMS Microbiol Rev 37 : 634 ~ 663
[학술지(정기간행물)] - Singh AK / 2014 / Rhizospheric fungal community structure of a Bt brinjal and a near isogenic variety / J Appl Microbiol 117 : 750 ~ 765
[학술지(정기간행물)] - Kristin A / 2013 / The root microbiotada fingerprint in the soil? / Plant Soil 370 : 671 ~ 686
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Endophytic fungi harbored in Panax notoginseng : diversity and potential as biological control agents against host plant pathogens of root-rot disease
Journal of Ginseng Research
2017 .07
-
Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng
Journal of Ginseng Research
2016 .04
-
Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging
Journal of Ginseng Research
2021 .11
-
The potential of Panax notoginseng against COVID-19 infection
Journal of Ginseng Research
2023 .09
-
Anti-inflammatory and Immunosuppressive Effects of Panax notoginseng
Natural Product Sciences
2019 .12
-
Endophytic Trichoderma gamsii YIM PH30019 : a promising biocontrol agent with hyperosmolar, mycoparasitism, and antagonistic activities of induced volatile organic compounds on root-rot pathogenic fungi of Panax notoginseng
Journal of Ginseng Research
2016 .10
-
Investigating chemical features of Panax notoginseng based on integrating HPLC fingerprinting and determination of multiconstituents by single reference standard
Journal of Ginseng Research
2018 .06
-
Analysis of common and characteristic actions of Panax ginseng and Panax notoginseng in wound healing based on network pharmacology and meta-analysis
Journal of Ginseng Research
2023 .07
-
Study on the grading standard of Panax notoginseng seedlings
Journal of Ginseng Research
2018 .04
-
UPLC-MS² combined molecular networking based discovery of nortriterpenoids from biotransformation of ginsenosides in Sanqi rhizosphere soil
Journal of Ginseng Research
2024 .11
-
Metabolomes and transcriptomes revealed the saponin distribution in root tissues of Panax quinquefolius and Panax notoginseng
Journal of Ginseng Research
2020 .11
-
Corrigendum to “Panax notoginseng saponins promotes angiogenesis after cerebral ischemia-reperfusion injury”[J Ginseng Res 48(6), November 2024, 592–602]
Journal of Ginseng Research
2025 .01
-
Stem-leaves of Panax as a rich and sustainable source of less-polar ginsenosides: comparison of ginsenosides from Panax ginseng, American ginseng and Panax notoginseng prepared by heating and acid treatment
Journal of Ginseng Research
2021 .01
-
Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detector
Journal of Ginseng Research
2018 .04
-
The effect of a novel botanical agent on early osteogenic response of osteoblasts
Oral Biology Research
2017 .01
-
전국 다중이용시설의 실내공기 부유 곰팡이의 현황 및 특성 분석
한국환경보건학회지
2022 .10
-
Dammarane-type triterpene oligoglycosides from the leaves and stems of Panax notoginseng and their antiinflammatory activities
Journal of Ginseng Research
2019 .06
-
Effects of cultivation ages and modes on microbial diversity in the rhizosphere soil of Panax ginseng
Journal of Ginseng Research
2016 .01
-
Mechanism of Panax notoginseng saponins modulation of miR-214-3p/NR1I3 affecting the pharmacodynamics and pharmacokinetics of warfarin
Journal of Ginseng Research
2024 .09
-
Investigation of the whitening activity of ginsenosides from Panax notoginseng and optimization of the dosage form
Journal of Ginseng Research
2024 .11
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2018-524-001598198