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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Persptectives Vol.6 No.6
- 발행연도
- 2015.1
- 수록면
- 336 - 340 (5page)
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Objectives: In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY-α- amylase. Methods: Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protein. Amylolytic activity of proteins produced by transformed E. coli cells was detected by zymography, and the rate of active α-amylase with and without the intein tag in both soluble conditions and as inclusion bodies solubilized by 4M urea were measured. Results: Amylolytic activity of ~185,000 U/L of bacterial culture was observed from the soluble form of the protein using this system. Conclusion: These results indicate that this expression system was appropriate for the production of thermostable α-amylase.
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참고문헌 (22)
참고문헌 신청
Zeng F / 2000 / Comparison of alpha-amylase and protease activities of a zoophytophagous and two phytozoophagous Heteroptera / Comp Biochem Physiol A Mol Integr Physiol 126(1):101~106
Matsuzaki H / 1974 / Hybrid alphaamylases produced by transformants of Bacillus subtilis. I. Purification and characterization of extracellular alpha-amylases produced by the parental strains and transformants / Biochim Biophys Acta 365(1):235~247
Yuuki T / 1985 / Complete nucleotide sequence of a gene coding for heat- and pH-stable alpha-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying alpha-amylases deduced from the DNA sequences / J Biochem 98(5):1147~1156
Saito N / 1973 / A thermophilic extracellular a-amylase from Bacillus licheniformis / Arch Biochem Biophys 155(2):290~298
Violet M / 1989 / Kinetic study of the irreversible thermal denaturation of Bacillus licheniformis alpha-amylase / Biochem J 263(3):665~670
Matsuzaki H / 1974 / Hybrid alphaamylases produced by transformants of Bacillus subtilis. I. Purification and characterization of extracellular alpha-amylases produced by the parental strains and transformants / Biochim Biophys Acta 365(1):235~247
Yuuki T / 1985 / Complete nucleotide sequence of a gene coding for heat- and pH-stable alpha-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying alpha-amylases deduced from the DNA sequences / J Biochem 98(5):1147~1156
Saito N / 1973 / A thermophilic extracellular a-amylase from Bacillus licheniformis / Arch Biochem Biophys 155(2):290~298
Violet M / 1989 / Kinetic study of the irreversible thermal denaturation of Bacillus licheniformis alpha-amylase / Biochem J 263(3):665~670
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