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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 563 - 571 (9page)
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초록· 키워드
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Purpose: Recent evidence shows that nitric oxide (NO) may exhibit both pro-cancer and anti-cancer activities. The present study aimed to determine the differentially expressedproteins in NO-treated NIH/3T3 fibroblasts in order to investigate whether NO induces proteins with pro-cancer or anti-cancer effects. Materials and Methods:The cells were treated with 300 μM of an NO donor 3,3-bis-(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18) for 12 h. The changed protein patterns, which were separated by two-dimensional electrophoresis using pH gradients of 4‒7, were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. Results: Seventeendifferentially expressed proteins were identified in NOC-18-treated cells. Nine proteins [vinculin protein, keratin 19, ubiquitous tropomodulin, F-actin capping protein (α1 subunit), tropomyosin 3, 26S proteasome-associated pad1 homolog, T-complex protein 1 (ε subunit) NG-dimethylarginine dimethylaminohydrolase, and heat shock protein 90] were increased and eight proteins (heat shock protein 70, glucosidaseII, lamin B1, calreticulin, nucleophosmin 1, microtubule-associated protein retinitis pigmentosa/end binding family member 1, 150 kD oxygen-regulated protein precursor, and heat shock 70-related protein albino or pale green 2) were decreased by NOC-18 in the cells. Thirteen proteins are related to the suppression of cancer cell proliferation, invasion, and metastasis while two proteins (heat shock protein 90 and NG-dimethylarginine dimethylaminohydrolase) are related to carcinogenesis. The functions of 150 kD oxygen-regulated protein precursor and T-complex protein 1 (ε subunit) are unknown in relation to carcinogenesis. Conclusion: Most proteins differentiallyexpressed by NOC-18 are involved in inhibiting cancer development.
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참고문헌 (25)
참고문헌 신청
Beck KF / 1999 / Inducible NO synthase: role in cellular signalling / J Exp Biol 202 (Pt 6) : 645 ~ 653
Brantley EC / 2010 / Nitric oxide-mediated tumoricidal activity of murine microglial cells / Transl Oncol 3 : 380 ~ 388
Carneiro ZA / 2014 / Nitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells / J Inorg Biochem 134 : 36 ~
Davies CM / 2008 / Reactive nitrogen and oxygen species in interleukin-1-mediated DNA damage associated with osteoarthritis / Osteoarthritis Cartilage 16 : 624 ~ 630
Faure O / 2004 / Inducible Hsp70 as target of anticancer immunotherapy: Identification of HLA-A*0201-restricted epitopes / Int J Cancer 108 : 863 ~ 870
Brantley EC / 2010 / Nitric oxide-mediated tumoricidal activity of murine microglial cells / Transl Oncol 3 : 380 ~ 388
Carneiro ZA / 2014 / Nitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells / J Inorg Biochem 134 : 36 ~
Davies CM / 2008 / Reactive nitrogen and oxygen species in interleukin-1-mediated DNA damage associated with osteoarthritis / Osteoarthritis Cartilage 16 : 624 ~ 630
Faure O / 2004 / Inducible Hsp70 as target of anticancer immunotherapy: Identification of HLA-A*0201-restricted epitopes / Int J Cancer 108 : 863 ~ 870
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