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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 387 - 397 (11page)
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Background: Three-dimensional (3D) in vitro cultures recapitulate the physiological microenvironment and exhibit high concordance with in vivo conditions. Improving co-culture models with different kind of cell types cultured on a 3D scaffold can closely mimic the in vivo environment. In this study, we examined the osteogenic response of pre-osteoblast MC3T3-E1 cells and Raw264.7 mouse monocytes in a 3D-encapsulated co-culture environment composed of the Cellrix® 3D culture system, which provides a physiologically relevant environment. Methods: The Cellrix® 3D Bio-Gel scaffolds were used to individually culture or co-culture two type cells in 3D microenvironment. Under 3D culture conditions, osteoblastic behavior was evaluated with an ALP assay and staining. ACP assay and TRAP staining were used as osteoclastic behavior indicator. Results: Treatment with osteoblastic induction factors (+3F) and RANKL had on positively effect on alkaline phosphatase activity but significantly inhibited to acid phosphatase activity during osteoclastic differentiation in 3D co-culture. Interestingly, alkaline phosphatase activity or acid phosphatase activity in 3D co-culture was stimulated with opposite differentiation factors at an early stage of differentiation. We guess that these effects may be related to RANK–RANKL signaling, which is important in osteoblast regulation of osteoclasts. Conclusion: In this study, the osteogenic response of 3D encapsulated pre-osteoblast MC3T3-E1 cells and mouse monocyte Raw264.7 cells was successfully demonstrated. Our 3D culture conditions will be able to provide a foundation for developing a high-throughput in vitro bone model to study the effects of various drugs and other agents on molecular pathways.
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참고문헌 (32)
참고문헌 신청
Rijal G / 2017 / Application of synthetic polymeric scaffolds in breast cancer 3D tissue cultures and animal tumor models / Int J Biomater 2017:8074890
Torricelli P / 2004 / In vitro models to test orthopedic biomaterials in view of their clinical application in osteoporotic bone / Int J Artif Organs 27:658~663
Chinta MS / 2020 / ‘‘Tissues in a dish’’: a review of organoids in plastic surgery / Plast Reconstr Surg Glob Open 8:e2787
Elliott NT / 2011 / A review of three-dimensional in vitro tissue models for drug discovery and transport studies / J Pharm Sci 100:59~74
Huang G / 2017 / Functional and biomimetic materials for engineering of the three-dimensional cell microenvironment / Chem Rev 117:12764~12850
Torricelli P / 2004 / In vitro models to test orthopedic biomaterials in view of their clinical application in osteoporotic bone / Int J Artif Organs 27:658~663
Chinta MS / 2020 / ‘‘Tissues in a dish’’: a review of organoids in plastic surgery / Plast Reconstr Surg Glob Open 8:e2787
Elliott NT / 2011 / A review of three-dimensional in vitro tissue models for drug discovery and transport studies / J Pharm Sci 100:59~74
Huang G / 2017 / Functional and biomimetic materials for engineering of the three-dimensional cell microenvironment / Chem Rev 117:12764~12850
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