Clinicopathologic Significance of BRAF Mutation and Extracellular Signal Regulated Kinase 1/2 Expression in Patients With a Colorectal Adenocarcinoma

김형욱                                    /KIM; HYUNG OOK; 김범규; 차성재; 박용검; 이태진                                         /LeeTaeJin

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자료유형: 학술저널

저자정보: 김형욱 /KIM HYUNG OOK 김범규 차성재 박용검 이태진 /LeeTaeJin

저널정보: 대한대장항문학회 Annals of Coloproctology Annals of Coloproctolgy Vol.31 No.1

발행연도: 2015.1

수록면: 9 - 15 (7page)

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Purpose: BRAF mutation and expression of extracellular signal regulated kinase (ERK) are linked with colorectal carcinogenesis through the serrated pathway. BRAF and ERK1/2 play important roles in the activation of mitogen-activated protein (MAP) kinase signaling pathways. The present study investigated the clinicopathologic outcomes of BRAF mutation and ERK1/2 expression in patients with colorectal cancer (CRC) and the possibility of using them as prognostic indicators. Methods: Dual-priming oligonucleotide-based multiplex polymerase chain reaction for BRAFV600E mutation and immunohistochemical analysis of ERK1/2 were performed using 65 formalin-fixed, paraffin-embedded samples from patients with CRC. We analyzed the dependences of the clinicopathologic features on BRAF mutation and ERK1/2 expression. Results: Out of 65 samples from CRC patients, BRAF mutation was detected in 3 (4.6%). The 3 patients with BRAF mutation presented with T3 CRC with lymph node metastasis (stage III) showing moderately or poorly differentiated histology. ERK1 and ERK2 were positively detected in 73.8% and 15.4% of the patients with CRC, respectively. ERK1 expression was significantly correlated with lymph node metastasis (P = 0.049). ERK2 expression was significantly correlated with tumor emboli (P < 0.05), tumor invasion (P = 0.035), lymph node metastasis (P = 0.017), and stage (P = 0.02). Conclusion: BRAF mutation and ERK1/2 expression may be associated with advanced or more aggressive CRC. These molecular markers might play prognostic roles in CRC developed through the serrated pathway.

#BRAF #ERK1 #ERK2 #Colorectal neoplasms #Adenocarcinoma

참고문헌 (32)

참고문헌 신청

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Yang S / 2004 / BRAF and KRAS Mutations in hyperplastic polyps and serrated adenomas of the colorectum: relationship to histology and CpG island methylation status / Am J Surg Pathol 28:1452~1459

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