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자료유형
학술저널
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한국키틴키토산학회 Journal of Chitin and Chitosan Journal of Chitin and Chitosan Vol.20 No.1
발행연도
2015.1
수록면
10 - 19 (10page)

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In this study, we investigated anti-neuroinflammatory activities of enzymatic extracts of the microalgae residues, which werederived from the lipid extraction process, to improve the economic feasibility of algal biofuel. After lipid extraction, three microalgalresidues (Chlorella sp., Nannochloropsis sp., Phaeodactylum tricornutum) were enzymatically hydrolyzed by four carbohydrases(Celluclast 1.5 L, Dextrozyme DX 1.5X, Promozyme D2, and Viscozyme L). Antioxidative activities of various enzymaticextracts from algal residues evaluated by measuring 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl,1,1-diphenyl-2-picrylhydrazyl (DPPH) and alkyl radical-scavenging activities. All enzymatic extracts had scavenging potenciesto ABTS+and alkyl radicals. Among the enzymatic extracts, Celluclast 1.5 L extracts from Nannochloropsis sp. residues(CN) showed the highest inhibitory activities on LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) inmurine BV-2 microglia, and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dosedependentmanner. CN also significantly reduced the generation of proinflammatory cytokines, such as interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor (TNF)-α in a dose-dependent manner. Hence, these antioxidant and anti-neuroinflammatoryeffects of CN might assist potential of microalgae residues as nutraceuticals for treatment and prevention againstneurodegenerative diseases that are accompanied by microglial activation.

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