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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Persptectives Vol.7 No.6
- 발행연도
- 2016.1
- 수록면
- 373 - 377 (5page)
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초록· 키워드
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Objectives: Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract pathogens can be extremely important. The aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (PCR) for rapid and simultaneous detection of Salmonella spp., Shigella spp., and Vibrio cholera. Methods: The standard and clinical strains of Salmonella spp., Shigella spp., and V. cholerae were used in the assay. Multiplex PCR was performed and optimized based on amplification of invA, putative integrase, and ompW genes for detecting Salmonella spp., Shigella spp., and V. cholerae, respectively. The specificity of the assay was evaluated by testing 12 different bacterial species. Results: Only Salmonella spp., Shigella spp., and V. cholerae strains had positive results when subjected to the assay using multiplex PCR. The assay showed a high sensitivity, and no amplification products were observed in multiplex PCR with any of the other microorganisms. Conclusion: Our study indicated that the invA, putative integrase, and ompWbased multiplex PCR assay appears to be an efficient method for rapid and simultaneous detection of Salmonella spp., Shigella spp., and V. cholerae.
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참고문헌 (35)
참고문헌 신청
Guerrant RL / 1990 / Diarrhea in developed and developing countries: magnitude, special settings, and etiologies / Rev Infect Dis 12:S41~S50
Ranjbar R / 2014 / Development of multiplex PCR for simultaneous detection of three pathogenic Shigella species / Iran J Public Health 43:1657~1663
Ranjbar R / 2014 / Use of TaqMan real-time PCR for rapid detection of Salmonella serotypes Typhi and Typhimurium / Acta Microbiol Immunol Hung 61:121~130
Mulatu G / 2014 / Prevalence of Shigella, Salmonella and Campylobacter species and their susceptibility patters among under five children with diarrhea in Hawassa town, south Ethiopia / Ethiop J Health Sci 24:101~108
Akoachere JFT / 2014 / Water sources as reservoirs of Vibrio cholerae O1 and non-O1 strains in Bepanda, Douala (Cameroon): relationship between isolation and physico-chemical factors / BMC Infect Dis 14:421
Ranjbar R / 2014 / Development of multiplex PCR for simultaneous detection of three pathogenic Shigella species / Iran J Public Health 43:1657~1663
Ranjbar R / 2014 / Use of TaqMan real-time PCR for rapid detection of Salmonella serotypes Typhi and Typhimurium / Acta Microbiol Immunol Hung 61:121~130
Mulatu G / 2014 / Prevalence of Shigella, Salmonella and Campylobacter species and their susceptibility patters among under five children with diarrhea in Hawassa town, south Ethiopia / Ethiop J Health Sci 24:101~108
Akoachere JFT / 2014 / Water sources as reservoirs of Vibrio cholerae O1 and non-O1 strains in Bepanda, Douala (Cameroon): relationship between isolation and physico-chemical factors / BMC Infect Dis 14:421
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