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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 751 - 760 (10page)
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초록· 키워드
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BACKGROUND: Bone tissue engineering based on pluripotent stem cells (PSCs) is a new approach to deal with bone defects. Protocols have been developed to generate osteoblasts from PSCs. However, the low efficiency of this process is still an important issue that needs to be resolved. Many studies have aimed to improve efficiency, but developing accurate methods to determine efficacy is also critical. Studies using pluripotency to estimate efficacy are rare. Telomerase is highly associated with pluripotency. METHODS: We have described a quantitative method to measure telomerase activity, telomeric repeat elongation assay based on quartz crystal microbalance (QCM). To investigate whether this method could be used to determine the efficiency of in vitro osteogenic differentiation based on pluripotency, we measured the pluripotency pattern of cultures through stemness gene expression, proliferation ability and telomerase activity, measured by QCM. RESULTS: We showed that the pluripotency pattern determined by QCM was similar to the patterns of proliferation ability and gene expression, which showed a slight upregulation at the late stages, within the context of the general downregulation tendency during differentiation. Additionally, a comprehensive gene expression pattern covering nearly every stage of differentiation was identified. CONCLUSION: Therefore, this assay may be powerful tools for determining the efficiency of differentiation systems based on pluripotency. In this study, we not only introduce a new method for determining efficiency based on pluripotency, but also provide more information about the characteristics of osteogenic differentiation which help facilitate future development of more efficient protocols.
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참고문헌 (31)
참고문헌 신청
Mateizel I / 2008 / Efficient differentiation of human embryonic stem cells into a homogeneous population of osteoprogenitor-like cells / Reprod Biomed Online 16:741~753
Duplomb L / 2007 / Concise review : embryonic stem cells : a new tool to study osteoblast and osteoclast differentiation / Stem Cells 25:544~552
Phillips BW / 2001 / Compactin enhances osteogenesis in murine embryonic stem cells / Biochem Biophys Res Commun 284:478~484
Langenbach F / 2013 / Effects of dexamethasone, ascorbic acid and b-glycerophosphate on the osteogenic differentiation of stem cells in vitro / Stem Cell Res Ther 4:117
Lou X / 2015 / Induced pluripotent stem cells as a new strategy for osteogenesis and bone regeneration / Stem Cell Rev 11:645~651
Duplomb L / 2007 / Concise review : embryonic stem cells : a new tool to study osteoblast and osteoclast differentiation / Stem Cells 25:544~552
Phillips BW / 2001 / Compactin enhances osteogenesis in murine embryonic stem cells / Biochem Biophys Res Commun 284:478~484
Langenbach F / 2013 / Effects of dexamethasone, ascorbic acid and b-glycerophosphate on the osteogenic differentiation of stem cells in vitro / Stem Cell Res Ther 4:117
Lou X / 2015 / Induced pluripotent stem cells as a new strategy for osteogenesis and bone regeneration / Stem Cell Rev 11:645~651
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