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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 92 - 97 (6page)
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B cell leukemia/lymphoma 3 (Bcl3) plays a pivotal role in immune homeostasis, cellular proliferation, and cell survival, as a co-activator or co-repressor of transcription of the NF-κB family. Recently, it was reported that Bcl3 positively regulates pluripotency genes, including Oct4, in mouse embryonic stem cells (mESCs). However, the role of Bcl3 in the maintenance of pluripotency and self-renewal activity is not fully established. Here, we report the dynamic regulation of the proliferation, pluripotency, and self-renewal of mESCs by Bcl3 via an influence on Nanog transcriptional activity. Bcl3 expression is predominantly observed in immature mESCs, but significantly decreased during cell differentiation by LIF depletion and in mESC-derived EBs. Importantly, the knockdown of Bcl3 resulted in the loss of self-renewal ability and decreased cell proliferation. Similarly, the ectopic expression of Bcl3 also resulted in a significant reduction of proliferation, and the self-renewal of mESCs was demonstrated by alkaline phosphatase staining and clonogenic single cell-derived colony assay. We further examined that Bcl3-mediated regulation of Nanog transcriptional activity in mESCs, which indicated that Bcl3 acts as a transcriptional repressor of Nanog expression in mESCs. In conclusion, we demonstrated that a sufficient concentration of Bcl3 in mESCs plays a critical role in the maintenance of pluripotency and the self-renewal of mESCs via the regulation of Nanog transcriptional activity.
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참고문헌 (24)
참고문헌 신청
Suda Y / 1987 / Mouse embryonic stem cells exhibit indefinite proliferative potential / J Cell Physiol 133:197~201
Niwa H / 2007 / How is pluripotency determined and maintained? / Development 134:635~646
Chambers I / 2003 / Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells / Cell 113:643~655
Mitsui K / 2003 / The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells / Cell 113:631~642
Boyer LA / 2005 / Core transcriptional regulatory circuitry in human embryonic stem cells / Cell 122:947~956
Niwa H / 2007 / How is pluripotency determined and maintained? / Development 134:635~646
Chambers I / 2003 / Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells / Cell 113:643~655
Mitsui K / 2003 / The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells / Cell 113:631~642
Boyer LA / 2005 / Core transcriptional regulatory circuitry in human embryonic stem cells / Cell 122:947~956
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