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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 393 - 401 (9page)
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Delivery of stem cells with osteogenesis while enabling angiogenesis is important for vascularized bone tissue engineering. Here a three-dimensional (3D) co-culture system of dental pulp stem cells (DPSCs) and endothelial cells (ECs) was designed using porous microcarriers, and the feasibility of applying to bone tissue engineering was investigated in vitro. Highly porous spherical microcarriers made of degradable biopolymers were prepared with sizes of hundreds of micrometers. The microcarriers loaded with DPSCs were co-cultured with ECs embedded in a hydrogel of type I collagen. An optimal coculture medium that preserves the viability of ECs while stimulating the osteogenic differentiation of DPSCs was found to be a 10:1 of osteogenic medium:endothelial medium. The co-cultured constructs of DPSCs/ECs showed significantly higher level of alkaline phosphatase activity than the mono-cultured cells. Moreover, the expressions of genes related with osteogenesis and angiogenesis were significantly up-regulated by the co-cultures with respect to the mono-cultures. Results imply the interplay between ECs and DPSCs through the designed 3D co-culture models. The microcarrier-enabled co-cultured cell system is considered to be useful as an alternative tool for future vascularized bone tissue engineering.
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참고문헌 (32)
참고문헌 신청
Langer R / 1993 / Tissue engineering / Science 260:920~926
Battiston KG / 2014 / Biomaterials in co-culture systems : towards optimizing tissue integration and cell signaling within scaffolds / Biomaterials 35:4465~4476
Nguyen TV / 2015 / Establishment of a hepatocyte–kupffer cell coculture model for assessment of proinflammatory cytokine effects on metabolizing enzymes and drug transporters / Drug Metab Dispos 43:774~785
Buitinga M / 2016 / Coculturing human islets with proangiogenic support cells to improve islet revascularization at the subcutaneous transplantation site / Tissue Eng Part A 22:375~385
Hertz J / 2013 / A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells / Acta Biomater 9:7622~7629
Battiston KG / 2014 / Biomaterials in co-culture systems : towards optimizing tissue integration and cell signaling within scaffolds / Biomaterials 35:4465~4476
Nguyen TV / 2015 / Establishment of a hepatocyte–kupffer cell coculture model for assessment of proinflammatory cytokine effects on metabolizing enzymes and drug transporters / Drug Metab Dispos 43:774~785
Buitinga M / 2016 / Coculturing human islets with proangiogenic support cells to improve islet revascularization at the subcutaneous transplantation site / Tissue Eng Part A 22:375~385
Hertz J / 2013 / A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells / Acta Biomater 9:7622~7629
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