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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 242 - 250 (9page)
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초록· 키워드
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Adipose-derived stem cells (ADSCs) are an attractive source of material for mesenchymal stem cell research due to the abundance of adipose and relative ease of access compared with bone marrow. A key consideration for research is whether cell isolation methods can be improved, to reduce the process steps needed to isolate and expand cell material. In the current study, we used macroporous biopolymer microcarriers to isolate primary ADSCs. We found that the method was capable of isolating ADSCs that were subsequently capable of being transferred to culture dishes and expanded in vitro. Moreover, flow cytometry revealed that they expressed typical stem cell markers and were capable of undergoing tri-lineage differentiation. In summary, it is feasible to use biopolymer microcarriers for retrieval of viable ADSCs that retain identity markers of stem cell function.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Zuk PA / 2001 / Multilineage cells from human adipose tissue: implications for cell-based therapies / Tissue Eng 7:211~228
Zuk PA / 2002 / Human adipose tissue is a source of multipotent stem cells / Mol Biol Cell 13:4279~4295
Hong L / 2007 / 17-Beta estradiol enhances osteogenic and adipogenic differentiation of human adipose-derived stromal cells / Tissue Eng 13:1197~1203
Minteer DM / 2015 / Analysis of type II diabetes mellitus adipose-derived stem cells for tissue engineering applications / J Tissue Eng 6
Davies OG / 2015 / Isolation of adipose and bone marrow mesenchymal stem cells using CD29 and CD90 modifies their capacity for osteogenic and adipogenic differentiation / J Tissue Eng 6
Zuk PA / 2002 / Human adipose tissue is a source of multipotent stem cells / Mol Biol Cell 13:4279~4295
Hong L / 2007 / 17-Beta estradiol enhances osteogenic and adipogenic differentiation of human adipose-derived stromal cells / Tissue Eng 13:1197~1203
Minteer DM / 2015 / Analysis of type II diabetes mellitus adipose-derived stem cells for tissue engineering applications / J Tissue Eng 6
Davies OG / 2015 / Isolation of adipose and bone marrow mesenchymal stem cells using CD29 and CD90 modifies their capacity for osteogenic and adipogenic differentiation / J Tissue Eng 6
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