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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 740 - 749 (10page)
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Pluripotent stem cells can differentiate into many cell types including mature hepatocytes, and can be used in the development of new drugs, treatment of diseases, and in basic research. In this study, we established a protocol leading to efficient hepatic differentiation, and compared the capacity to differentiate into the hepatocyte lineage of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Optimal combinations of cytokines and growth factors were added to embryoid bodies produced by both types of cell. Differentiation of the cells was assessed with optical and electron microscopes, and hepatic-specific transcripts and proteins were detected by quantitative reverse transcription polymerase chain reaction and immunocytochemistry, respectively. Both types of embryoid body produced polygonal hepatocyte-like cells accompanied by time-dependent up regulation of genes for α-fetoprotein, albumin (ALB), asialoglycoprotein1, CK8, CK18, CK19, CYP1A2, and CYP3A4, which are expressed in fetal and adult hepatocytes. Both types of cell displayed functions characteristic of mature hepatocytes such as accumulation of glycogen, secretion of ALB, and uptake of indocyanine green. And these cells are transplanted into mouse model. Our findings indicate that hESCs and hiPSCs have similar abilities to differentiate into hepatocyte in vitro using the protocol developed here, and these cells are transplantable into damaged liver.
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참고문헌 (35)
참고문헌 신청
Park IH / 2008 / Reprogramming of human somatic cells to pluripotency with defined factors / Nature 451:141~146
Takahashi K / 2007 / Induction of pluripotent stem cells from adult human fibroblasts by defined factors / Cell 131:861~872
Hamazaki T / 2001 / Hepatic maturation in differentiating embryonic stem cells in vitro / FEBS Lett 497:15~19
Keller G / 1993 / Hematopoietic commitment during embryonic stem cell differentiation in culture / Mol Cell Biol 13:473~486
Lee SH / 2000 / Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells / Nat Biotechnol 18:675~679
Takahashi K / 2007 / Induction of pluripotent stem cells from adult human fibroblasts by defined factors / Cell 131:861~872
Hamazaki T / 2001 / Hepatic maturation in differentiating embryonic stem cells in vitro / FEBS Lett 497:15~19
Keller G / 1993 / Hematopoietic commitment during embryonic stem cell differentiation in culture / Mol Cell Biol 13:473~486
Lee SH / 2000 / Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells / Nat Biotechnol 18:675~679
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