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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 579 - 586 (8page)
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Target cells differentiation techniques from stem cells are developed rapidly. Recently, direct conversion techniques are introduced in various categories. Unlike pluripotent stem cells, this technique enables direct differentiation into the other cell types such as neurons, cardiomyocytes, insulin-producing cells, and hepatocytes without going through the pluripotent stage. However, the function of these converted cells reserve an immature phenotype. Therefore, we modified the culture conditions of mouse direct converted hepatocytes (miHeps) to mature fetal characteristics, such as higher AFP and lower albumin (ALB) expression than primary hepatocytes. First, we generate miHeps from mouse embryonic fibroblasts (MEFs) with two transcription factors HNF4a and Foxa3. These cells indicate typical epithelial morphology and express hepatic proteins. To mature hepatic function, DMSO is treated during culture time for more than 7 days. After maturation, miHeps showed features of maturation such as exhibiting typical hepatocyte-like morphology, increased up-regulated ALB and CYP enzyme gene expression, down-regulated AFP expressions, and acquired hepatic function over time. Thus, our data provides a simple method to mature direct converted hepatocytes functionally and these cells enable them to move closer to generating functional hepatocytes.
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참고문헌 (28)
참고문헌 신청
Wang FS / 2014 / The global burden of liver disease : the major impact of China / Hepatology 60:2099~2108
Vierbuchen T / 2010 / Direct conversion of fibroblast to functional neurons by defined factors / Nature 463:1035~1041
Pang ZP / 2011 / Induction of human neuronal cells by defined transcription factors / Nature 476:220~223
Song K / 2012 / Heart repair by reprogramming non-myocytes with cardiac transcription factors / Nature 485:599~604
Sekiya S / 2011 / Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors / Nature 475:390~393
Vierbuchen T / 2010 / Direct conversion of fibroblast to functional neurons by defined factors / Nature 463:1035~1041
Pang ZP / 2011 / Induction of human neuronal cells by defined transcription factors / Nature 476:220~223
Song K / 2012 / Heart repair by reprogramming non-myocytes with cardiac transcription factors / Nature 485:599~604
Sekiya S / 2011 / Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors / Nature 475:390~393
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