Malvidin Protects WI-38 Human Fibroblast Cells Against Stress-induced Premature Senescence

서혜린; 최미진; 최지명; 고종철; 고지연; 조은주

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자료유형: 학술저널

저자정보: 서혜린 (부산대학교) 최미진 (부산대학교) 최지명 (부산대학교) 고종철 (국립식량과학원) 고지연 (국립식량과학원) 조은주 (부산대학교)

저널정보: 대한암예방학회 대한암예방학회지 대한암예방학회지 제21권 제1호

발행연도: 2016.1

수록면: 32 - 40 (9page)

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Background: Malvidin is one of the most abundant components in red wines and black rice. The effects of malvidin on aging and lifespan under oxidative stress have not been fully understood. This study focused on the anti-aging effect of malvidin on stress-induced premature senescence (SIPS) in WI-38 human lung-derived diploid fibroblasts. Methods: In order to determine the viability of WI-38 cells, MTT assay was conducted, and malondialdehyde level was determined using thiobarbituric acid-reactive substance assay. Protein expression of inflammation-related factors was also evaluated by Western blot analysis. Results: Acute and chronic oxidative stress via hydrogen peroxide (H2O2) treatment led to SIPS in WI-38 cells, which showed decreased cell viability, increased lipid peroxidation, and a shortened lifespan in comparison with non-H2O2-treated WI-38 cells. However, malvidin treatment significantly attenuated H2O2-induced oxidative stress by inhibiting lipid peroxidation and increasing cell viability. Furthermore, the lifespan of WI-38 cells was prolonged by malvidin treatment. In addition, malvidin downregulated the expression of oxidative stress-related proteins, including NF-B, COX-2, and inducible nitric oxide synthase. Furthermore, protein expression levels of p53, p21, and Bax were also regulated by malvidin treatment in WI-38 cells undergoing SIPS. Conclusions: Malvidin may potentially inhibit the aging process by controlling oxidative stress.

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참고문헌 신청

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