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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.4
- 수록면
- 185 - 195 (11page)
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초록· 키워드
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Background: To investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male subfertility animal models.
Methods: Hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting.
Results: GINST treatment (50 μg/mL, 100 μg/mL, and 200 μg/mL) significantly (p < 0.05) inhibited the H2O2-induced (200 μM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 μg/mL and 100 μg/mL) significantly (p < 0.05) ameliorated the H<SUB>2</SUB>O<SUB>2</SUB>-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-α, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated H<SUB>2</SUB>O<SUB>2</SUB>-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats.
Conclusion: GINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility.
Methods: Hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting.
Results: GINST treatment (50 μg/mL, 100 μg/mL, and 200 μg/mL) significantly (p < 0.05) inhibited the H2O2-induced (200 μM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 μg/mL and 100 μg/mL) significantly (p < 0.05) ameliorated the H<SUB>2</SUB>O<SUB>2</SUB>-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-α, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated H<SUB>2</SUB>O<SUB>2</SUB>-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats.
Conclusion: GINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusion
References
참고문헌 (59)
참고문헌 신청
[학술지(정기간행물)] - Attele AS / 1999 / Ginseng pharmacology: multiple constituents and multiple actions / Biochem Pharmacol 58 : 1685 ~ 1693
[학술지(정기간행물)] - Kopalli SR / 2015 / Korean red ginseng extract rejuvenates testicular ineffectiveness and sperm maturation process in aged rats by regulating redox proteins and oxidative defense mechanisms / Exp Gerontol 69 : 94 ~ 102
[학술지(정기간행물)] - Ramesh T / 2012 / Effect of fermented Panax ginseng extract (GINST) on oxidative stress and antioxidant activities in major organs of aged rats / Exp Gerontol 47 : 77 ~ 84
[학술지(정기간행물)] - Won YJ / 2014 / Pectinase-treated Panax ginseng extract (GINST) rescues testicular dysfunction in aged rats via redox-modulating proteins / Exp Gerontol 53 : 57 ~ 66
[학술지(정기간행물)] - Choi HK / 1995 / Clinical efficacy of Korean red ginseng for erectile dysfunction / Int J Impot Res 7 : 181 ~ 186
[학술지(정기간행물)] - Kopalli SR / 2015 / Korean red ginseng extract rejuvenates testicular ineffectiveness and sperm maturation process in aged rats by regulating redox proteins and oxidative defense mechanisms / Exp Gerontol 69 : 94 ~ 102
[학술지(정기간행물)] - Ramesh T / 2012 / Effect of fermented Panax ginseng extract (GINST) on oxidative stress and antioxidant activities in major organs of aged rats / Exp Gerontol 47 : 77 ~ 84
[학술지(정기간행물)] - Won YJ / 2014 / Pectinase-treated Panax ginseng extract (GINST) rescues testicular dysfunction in aged rats via redox-modulating proteins / Exp Gerontol 53 : 57 ~ 66
[학술지(정기간행물)] - Choi HK / 1995 / Clinical efficacy of Korean red ginseng for erectile dysfunction / Int J Impot Res 7 : 181 ~ 186
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UCI(KEPA) : I410-ECN-0101-2018-524-001598269