Detection of O-Linked-N-Acetylglucosamine Modification and Its Associated Enzymes in Human Degenerated Intervertebral Discs

Georgios Nikolaou; Aristeidis H. Zibis; Apostolos H. Fyllos; Antonios Katsioulis; Sotirios Sotiriou; Anastasios Kotrotsios; Markos Sgantzos; Aikaterini Vassiou; Dimitrios L. Arvanitis

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자료유형: 학술저널

저자정보: Georgios Nikolaou (University of Thessaly) Aristeidis H. Zibis (University of Thessaly) Apostolos H. Fyllos (University of Thessaly) Antonios Katsioulis (University of Thessaly) Sotirios Sotiriou (University of Thessaly) Anastasios Kotrotsios (University of Thessaly) Markos Sgantzos (University of Thessaly) Aikaterini Vassiou (University of Thessaly) Dimitrios L. Arvanitis (University of Thessaly)

저널정보: 대한척추외과학회 Asian Spine Journal Asian Spine Journal Vol.11 No.6

발행연도: 2017.1

수록면: 863 - 869 (7page)

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Study Design: Human herniated discs were obtained from discectomy specimens for the immunohistochemical detection of OGlcNAc and O-GlcNAcase (OGA)/O-GlcNAc transferase (OGT). Purpose: This study aimed to quantify the extent of O-GlcNAcylation and its associated enzymes (OGT/OGA) in human degenerated intervertebral discs. Overview of Literature: The O-GlcNAcylation of nuclear, cytoplasmic, and mitochondrial proteins as well as the effects of such post-translational modifications are currently the focus of extensive research. O-GlcNAcylation is believed to contribute to the etiology of chronic illnesses by acting as a nutrient and stress sensor in the cellular environment. Mature intervertebral disc cells are chondrocyte-like cells, and O-GlcNAc has been shown to promote chondrocyte apoptosis in vitro . We believe that O-GlcNAcylation is a key regulator of disc degeneration. Methods: Fifty-six specimens were fixed for 24 hours in a 10% solution of neutral-buffered formaldehyde, dehydrated, and embedded in paraffin. Tissue slices (4-μm-thick) were used for hematoxylin-eosin staining and immunohistochemistry. Results: We found that O-GlcNAcylation of cytoplasmic proteins was less than that of nuclear proteins in both single cells and cell clusters. Cytoplasmic O-GlcNAcylation occurs subsequent to nuclear O-GlcNAcylation and is directly proportional to disc degeneration. OGT and O-GlcNAc expression levels were identical in all specimens examined. Conclusions: O-GlcNAc and OGA/OGT expression is shown to correlate for the first time with intervertebral disc cell degeneration. Increasing disc degeneration is associated with increasing O-GlcNAcylation in both nuclear and cytoplasmic proteins in human disc cells.

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