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논문 기본 정보

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학술저널
저자정보
Rajasekaran Shanmuganathan (Department of Spine Surgery Ganga Hospital Coimbatore India) Soundararajan Dilip Chand Raja (Department of Spine Surgery Ganga Hospital Coimb) Tangavel Chitraa (Ganga Research Centre Coimbatore India) K.S. Sri Vijay Anand (Department of Spine Surgery Ganga Hospital) Nayagam Sharon Miracle (Ganga Research Centre Coimbatore India) Matchado Monica Steffi (Ganga Research Centre Coimbatore India) Muthurajan Raveendran (Tamil Nadu Agricultural University Coimbatore) Shetty Ajoy Prasad (Department of Spine Surgery Ganga Hospital Coimb) Kanna Rishi Mugesh (Department of Spine Surgery Ganga Hospital Coimb) Dharmalingam Kuppamuthu (Aravind Medical Research Foundation)
저널정보
대한척추외과학회 Asian Spine Journal Asian Spine Journal Vol.14 No.4
발행연도
2020.1
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409 - 420 (12page)

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Study Design: Profiling proteins expressed in the nucleus pulposus of fetal intervertebral disc (IVD).Purpose: To evaluate the molecular complexity of fetal IVDs not exposed to mechanical, traumatic, inflammatory, or infective insults to generate improved knowledge on disc homeostasis.Overview of Literature: Low back pain is the most common musculoskeletal disorder, causing a significant reduction in the quality of life, and degenerative disc disorders mainly contribute to the increasing socioeconomic burden. Despite extensive research, the causative pathomechanisms behind degenerative disc disorders are poorly understood. Precise molecular studies on the intricate biological processes involved in maintaining normal disc homeostasis are needed.Methods: IVDs of nine fetal specimens obtained from medical abortions were used to dissect out the annulus fibrosus and nucleus pulposus under sterile operating conditions. Dissected tissues were transferred to sterile Cryovials and snap frozen in liquid nitrogen before transporting to the research laboratory for protein extraction and further liquid chromatography tandem mass spectrometry (LC-MS/ MS) analysis. Collected data were further analyzed using Gene Functional Classification Tool in DAVID and STRING databases.Results: A total of 1,316 proteins were identified through LC-MS/MS analysis of nine fetal IVD tissues. Approximately 247 proteins present in at least four fetal discs were subjected to further bioinformatic analysis. The following 10 clusters of proteins were identified: collagens, ribosomal proteins, small leucine-rich proteins, matrilin and thrombospondin, annexins, protein disulfide isomerase family proteins and peroxiredoxins, tubulins, histones, hemoglobin, and prolyl 4-hydroxylase family proteins.Conclusions: This study provides fundamental information on the proteome networks involved in the growth and development of healthy fetal discs in humans. Systematic cataloging of proteins involved in various structural and regulatory processes has been performed. Proteins expressed most abundantly (collagen type XIV alpha 1 chain, biglycan, matrilin 1, and thrombospondin 1) in their respective clusters also elucidate the possibility of utilizing these proteins for potential regenerative therapies.
#Intervertebral disc degeneration #Low back pain #Disc degeneration #Fetal intervertebral disc proteome

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참고문헌 (28)

참고문헌 신청
Schizas C / 2006 / Human adult and fetal intervertebral disc cell cultures: the way forward? / Orthop Proc 88-B(Supp_I):152 google schola Zybailov B / 2006 / Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae / J Proteome Res 5:2339~2347 google schola Choi KS / 2011 / Hedgehog signaling is required for formation of the notochord sheath and patterning of nuclei pulposi within the intervertebral discs / Proc Natl Acad Sci U S A 108:9484~9489 google schola Palmer A / 2003 / Multiple roles of ephrins in morphogenesis, neuronal networking, and brain function / Genes Dev 17:1429~1450 google schola Sakai D / 2003 / Transplantation of mesenchymal stem cells embedded in Atelocollagen gel to the intervertebral disc : a potential therapeutic model for disc degeneration / Biomaterials 24:3531~3541 google schola

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