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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 234 - 241 (8page)
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Background and Objectives: The benefit of high glucose-insulin-potassium (GIK) solution in clinical applications is controversial. We established a neonatal rat ventricular myocyte (NRVM) in vitro coverslip ischemia/reperfusion (I/R) model and investigated the effects of GIK solution on suppressing reactive oxygen species (ROS) and upregulating O -GlcNacylation, which protects cells from ischemic injury.
Materials and Methods: NRVMs were isolated from postnatal day 3–4 Sprague–Dawley rat pups and grown in Dulbecco’s modified Eagle’s medium containing high glucose (4.5 g/L), fetal bovine serum, and penicillin/streptomycin. The effects of the GIK solution on ROS production, apoptosis, and expression of O -GlcNAc and O -GlcNAc transferase (OGT) were investigated in the coverslip I/R model.
Results: Covering the 24-well culture plates for 3 hr with 12 mm diameter coverslips resulted in the appropriate ischemic shock. Glucose and insulin synergistically reduced ROS production, protected NRVM dose-dependently from apoptosis, and altered O -GlcNAc and OGT expression.
Conclusion: The high GIK solution protected NRVM from I/R injury in vitro by reducing ROS and altering O- GlcNacylation.
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참고문헌 (30)
참고문헌 신청
Yellon DM / 2007 / Myocardial reperfusion injury / N Engl J Med 357:1121~1135
Murry CE / 1986 / Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium / Circulation 74:1124~1136
Thibault H / 2007 / Postconditioning the human heart / Heart Metab 37:19~22
Jovic M / 2009 / Preconditioning with glucose-insulin-potassium solution and restoration of myocardial function during coronary surgery / Gen Physiol Biophys 28:262~270
Sodi-Pallares D / 1962 / Effects of an intravenous infusion of a potassium-glucose-insulin solution on the electrocardiographic signs of myocardial infarction. A preliminary clinical report / Am J Cardiol 9:166~181
Murry CE / 1986 / Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium / Circulation 74:1124~1136
Thibault H / 2007 / Postconditioning the human heart / Heart Metab 37:19~22
Jovic M / 2009 / Preconditioning with glucose-insulin-potassium solution and restoration of myocardial function during coronary surgery / Gen Physiol Biophys 28:262~270
Sodi-Pallares D / 1962 / Effects of an intravenous infusion of a potassium-glucose-insulin solution on the electrocardiographic signs of myocardial infarction. A preliminary clinical report / Am J Cardiol 9:166~181
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