지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 25 - 30 (6page)
이용수
초록· 키워드
오류제보하기
Background: Mesenchymal stem cells (MSCs) play an important role in hematopoietic stem cell (HSC) maintenance, proliferation, and apoptosis. DNA methyltransferase 1 (DNMT1) is consid-ered an essential factor in the maintenance of HSCs in mammalian cells. Therefore, this study was conducted to evaluate the mRNA expression level of DNMT1 during cord blood (CB)-HSC ex vivo expansion with MSCs. Methods: Ex vivo cultures of CB-HSCs were performed in three culture conditions for 7 days: cyto-kines, cytokines with MSCs, and only MSCs. Total and viable cell numbers were counted after 5 and 7 days using trypan blue stain, and the stem cell percentage was then evaluated by flow cytometry. Moreover, in vitro colony-forming unit assay was carried out to detect clonogenic potential of HSCs at days 0 and 7 using MethoCult H4434. Finally, DNMT1 mRNA expression level was evaluated by real-time polymerase chain reaction. Results: Maximum CB-CD34＋ cell expansion was observed on day 7 in all the three cultures. After 7 days, ex vivo expansion of CB-CD34＋ cells indicated a significant decrease in DNMT1 expression in the cytokine cultures, whereas in the two co-culture conditions DNMT1 ex-pression was increased. A significant difference between the number of CD34＋ and CD34−cells in the cytokine co-culture system was observed. Conclusion: These data indicated that an elevated expression of DNMT1 is associated with increased expansion and proliferation of HSCs co-cultured with human MSCs. Hence, DNMT1 may be a potential factor in the maintenance of expanded HSCs co-cultured with human MSCs.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Delaney C / 2010 / Strategies to enhance umbilical cord blood stem cell engraftment in adult patients / Expert Rev Hematol 3:273~283
Broxmeyer HE / 2010 / Umbilical cord transplantation : epilogue / Semin Hematol 47:97~103
Dahlberg A / 2011 / Ex vivo expansion of human hematopoietic stem and progenitor cells / Blood 117:6083~6090
Conrad PD / 1998 / Ex vivo expansion of hematopoietic cells from umbilical cord blood for clinical transplantation / J Leukoc Biol 64:147~155
Alenzi FQ / 2009 / The haemopoietic stem cell : between apoptosis and self renewal / Yale J Biol Med 82:7~18
Broxmeyer HE / 2010 / Umbilical cord transplantation : epilogue / Semin Hematol 47:97~103
Dahlberg A / 2011 / Ex vivo expansion of human hematopoietic stem and progenitor cells / Blood 117:6083~6090
Conrad PD / 1998 / Ex vivo expansion of hematopoietic cells from umbilical cord blood for clinical transplantation / J Leukoc Biol 64:147~155
Alenzi FQ / 2009 / The haemopoietic stem cell : between apoptosis and self renewal / Yale J Biol Med 82:7~18
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Inhibition of hepatic stellate cells by bone marrow-derived mesenchymal stem cells in hepatic fibrosis
Clinical and Molecular Hepatology
2015 .01
-
Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement- free monolayer culture method
Blood Research
2017 .01
-
Sperm chromatin and DNA integrity, methyltransferase mRNA levels, and global DNA methylation in oligoasthenoteratozoospermia
Clinical and experimental reproductive medicine : CERM
2018 .01
-
Progress and emerging techniques for biomaterial-based derivation of mesenchymal stem cells (MSCs) from pluripotent stem cells (PSCs)
생체재료학회지
2023 .03
-
Immune Tolerance of Human Dental Pulp-Derived Mesenchymal Stem Cells Mediated by CD4+CD25+ FoxP3+ Regulatory T-Cells and Induced by TGF-β1 and IL-10
Yonsei Medical Journal
2017 .01
-
호흡기 질환에서의 중간엽 줄기 세포의 치료
대한내과학회지
2015 .01
-
DNA Methyltransferase Gene Polymorphisms for Prediction of Radiation-Induced Skin Fibrosis after Treatment of Breast Cancer: A Multifactorial Genetic Approach
Cancer Research and Treatment
2017 .01
-
In adipocytes, DNMT1 is an epigenetic safeguard of mitochondrial dynamics
한국실험동물학회 학술발표대회 논문집
2022 .07
-
Establishment of bovine tongue epithelium-derived mesenchymal stem cells
예방수의학회지
2017 .09
-
Methyl Donor Status Influences DNMT Expression and Global DNA Methylation in Cervical Cancer Cells
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Differentiation potential of mesenchymal stem cells through electrical stimulation
예방수의학회지
2019 .01
-
Advanced Research on Stem Cell Therapy for Hepatic Diseases: Potential Implications of a Placenta-derived Mesenchymal Stem Cell-based Strategy
Hanyang Medical Reviews
2015 .01
-
The advantages and limitations of mesenchymal stem cells in clinical application for treating human diseases
Osteoporosis and Sarcopenia
2018 .01
-
Dose- and sex-dependent effects on umbilical cord-derived mesenchymal stem cell efficacy in regeneration of a full- thickness tendon defect in a rat model
대한견주관절의학회지
2025 .03
-
Isolation and characterization of deer-derived mesenchymal stem cells
예방수의학회지
2019 .01
-
Characteristics of DNMT3A mutations in acute myeloid leukemia
Blood Research
2020 .01
-
DNMT3B -149 C>T and -579 G>T Polymorphisms and Risk of Gastric and Colorectal Cancer: a Meta-analysis
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Adult Stem Cell Treatment for Rheumatoid Arthritis
대한류마티스학회지
2018 .01
-
The autophagy Protein Atg5 Plays a Crucial Role in the Maintenance and Reconstitution Ability of Hematopoietic Stem Cells
Immune Network
2019 .04
-
First identified Korean family with Tatton-Brown-Rahman Syndrome caused by the novel DNMT3A variant c.118G>C p.(Glu40Gln)
Annals of Pediatirc Endocrinology & Metabolism
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0