Evaluation of zinc finger E-box binding homeobox 1 and transforming growth factor-beta2 expression in bladder cancer tissue in comparison with healthy adjacent tissue

Ali Mahdavinezhad; Reza Yadegarazari; Seyed Habibollah Mousavi-Bahar; Jalal Poorolajal; Mohammad Jafari; Mohammad Ali Amirzargar; Hosein Effatpanah; Massoud Saidijam

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자료유형: 학술저널

저자정보: Ali Mahdavinezhad (Hamadan University of Medical Sciences) Reza Yadegarazari (Hamadan University of Medical Sciences) Seyed Habibollah Mousavi-Bahar (Hamadan University of Medical Sciences) Jalal Poorolajal (Hamadan University of Medical Sciences) Mohammad Jafari (Hamadan University of Medical Sciences) Mohammad Ali Amirzargar (Hamadan University of Medical Sciences) Hosein Effatpanah (Hamadan University of Medical Sciences) Massoud Saidijam (Hamadan University of Medical Sciences)

저널정보: 대한비뇨기과학회 Investigative and Clinical Urology Investigative and Clinical Urology Vol.58 No.2

발행연도: 2017.1

수록면: 140 - 145 (6page)

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Purpose: The fifth most common cancer is allocated to bladder cancer (BC) worldwide. Understanding the molecular mechanisms of BC invasion and metastasis to identify target therapeutic strategies will improve disease survival. So the aim of this study was to measure expression rate of zinc finger E-box binding homeobox 1 (ZEB1) and transforming growth factor-beta2 (TGF-β2) mRNA in tissue samples of patients with BC and its healthy adjacent tissue samples and their association with muscle invasion, size and grade of the tumor. Materials and Methods: Tissue samples were collected from 35 newly diagnosed untreated patients with BC from 2013 to 2014. Total RNA was extracted from about 50-mg tissue samples using TRIzol reagent. TAKARA SYBR Premix EX Tag II was applied to determine the rate of mRNA expression by real-time polymerase chain reaction (PCR). To obtain final validation, PCR product of ZEB1 and TGF-β2 were sequenced. STATA 11 software was used to analyze the data. Results: The expression level of ZEB1 in tumor samples was significantly more than of in healthy adjacent tissue samples. Up-regulation of TGF-β2 showed a strong association with muscle invasion (p=0.017). There was also demonstrated a relationship between over expression of ZEB1 with the tumor size (p=0.050). Conclusions: It looks ZEB1 and TGF-β2 had a role in BC patients. In this study ZEB1 expression was higher in BC tissues than that of in healthy control tissues. There was demonstrated a markedly association between overexpression of TGF-β2 and muscle invasion. Therefore, they are supposed to be candidate as potential biomarkers for early detection and progression of BC.

참고문헌 (24)

참고문헌 신청

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Mousavi SM / 2012 / Guideline: National cancer registry (persian). 2nd ed. Tehran (IR): Ministry of health and medical education,office of Deputy minister for health, center for disease control and prevention,cancer office

Adam L / 2009 / MiR-200 expression regulates epithelial-to-mesenchymal transition in bladder cancer cells and reverses resistance to epidermal growth factor receptor therapy / Clin Cancer Res 15:5060~5072

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Wu K / 2013 / Silibinin inhibits β-catenin/ZEB1 signaling and suppresses bladder cancer metastasis via dual-blocking epithelial-mesenchymal transition and stemness / Cell Signal 25:2625~2633

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