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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 1,209 - 1,222 (14page)
- DOI
- 10.1007/s13258-021-01143-7
이용수
초록· 키워드
오류제보하기
Background Overexpression of the abiotic and biotic stress-resistance genes of the plant signaling pathway is well known for its signifcant role in the regulation of plant growth and enhancement of the productivity of agricultural land under changing climatic conditions.
Objectives This research aimed to clone Populus davidiana×Populus bolleana PP2C (PdPP2C) gene and analyze its structure and function, and downregulate PdPP2C by overexpression of its antisense PdPP2C (AS-PdPP2C) gene for enhancing cold resistance in transgenic lines of hybrid P. davidiana×P. bolleana.
Methods PdPP2C was cloned and transformed to identify its function, and its antisense was overexpressed via downregulation to increase the cold resistance in transgenic lines of hybrid P. davidiana×P. bolleana.
Results Antisense inhibition of protein phosphatase 2C accelerates the cold acclimation of Poplar (P. davidiana×P. bolleana) gene in terms of antifreeze.
Conclusion PdPP2C was expressed in the roots, stems, and leaves of P. davidiana×P. bolleana, and the expression was higher in the leaves. The expression of PdPP2C was also signifcantly downregulated at low-temperature (0 °C and 4 °C) stress. The relative conductivity and malondialdehyde content of non-transgenic lines were higher than those of AS-PdPP2C lines after 2 days of cold treatment at−1 °C. The leaves of the transgenic lines were not wilted and showed no chlorosis compared with those of the non-transgenic lines. The AS-PdPP2C transgenic lines also showed higher freezing resistance than the non-transgenic lines. AS-PdPP2C participated in the regulation of freezing resistance.
목차
등록된 정보가 없습니다.
참고문헌 (44)
참고문헌 신청
Bhaskara GB / 2019 / The fip side of phospho-signalling : regulation of protein dephosphorylation and the protein phosphatase 2Cs / Plant Cell Environ 42(10):2913~2930
Cohen P / 1989 / Protein phosphatases come of age / J Biol Chem 264(36):21435~21438
Feng J / 2019 / TaSnRK2. 9, a sucrose non-fermenting 1-related protein kinase gene, positively regulates plant response to drought and salt stress in transgenic tobacco / Front Plant Sci 9:2003
Feng X / 2019 / TaEXPB7-B, a β-expansin gene involved in low-temperature stress and abscisic acid responses, promotes growth and cold resistance in Arabidopsis thaliana / J Plant Physiol 240:153004
He F / 2018 / Pe CHYR 1, a ubiquitin E3 ligase from Populus euphratica, enhances drought tolerance via ABA-induced stomatal closure by ROS production in Populus / Plant Biotechnol J 16(8):1514~1528
Cohen P / 1989 / Protein phosphatases come of age / J Biol Chem 264(36):21435~21438
Feng J / 2019 / TaSnRK2. 9, a sucrose non-fermenting 1-related protein kinase gene, positively regulates plant response to drought and salt stress in transgenic tobacco / Front Plant Sci 9:2003
Feng X / 2019 / TaEXPB7-B, a β-expansin gene involved in low-temperature stress and abscisic acid responses, promotes growth and cold resistance in Arabidopsis thaliana / J Plant Physiol 240:153004
He F / 2018 / Pe CHYR 1, a ubiquitin E3 ligase from Populus euphratica, enhances drought tolerance via ABA-induced stomatal closure by ROS production in Populus / Plant Biotechnol J 16(8):1514~1528
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