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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 237 - 242 (6page)
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AT-hook proteins of plant have shown to be involved in growth and development through the modification of chromatin architecture to co-regulate transcription of genes. Recently, many genes encoding AT-hook protein have been identified and their involvement in senescence delay is investigated. In this study, soybean transgenic plants overexpressing chromatin architecturecontrolling ATPG7 gene was produced by Agrobacterium-mediated transformation and investigated for the positive effect on the important agronomic traits mainly focusing on yield-related components. A total of 27 transgenic soybean plants were produced from about 400 explants. T1 seeds were harvested from all transgenic plants. In the analysis of genomic DNAs from soybean transformants, ATPG7 and Bar fragments were amplified as expected, 975 bp and 408 bp in size, respectively. And also exact gene expression was confirmed by reverse transcriptase-PCR (RT-PCR) from transgenic line #6, #7 and #8. In a field evaluation of yield components of ATPG7 transgenic plants (T3), higher plant height, more of pod number and greater average total seed weight were observed with statistical significance. The results of this study indicate that the introduction of ATPG7 gene in soybean may have the positive effect on yield components.
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참고문헌 (16)
참고문헌 신청
Hinchee MAW / 1988 / Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer / Nat. Biotechnol 6:915~922
John KMM / 2016 / Metabolite changes in nine different soybean varieties grown under field and greenhouse conditions / Food Chem 211:347~355
Karimi M / 2002 / GatewayTM vectors for Agrobacterium-mediated plant transformation / Trends Plant Sci 7:193~195
김혜정 / 2016 / RNAi-mediated Soybean mosaic virus (SMV) resistance of a Korean Soybean cultivar / Plant Biotechnology Reports 10(5):257~267
Hye Jeong Kim / 2013 / Characterization of SMV resistance of soybean produced by genetic transformation of SMV-CP gene in RNAi / Plant Biotechnology Reports 7(4):425~433
John KMM / 2016 / Metabolite changes in nine different soybean varieties grown under field and greenhouse conditions / Food Chem 211:347~355
Karimi M / 2002 / GatewayTM vectors for Agrobacterium-mediated plant transformation / Trends Plant Sci 7:193~195
김혜정 / 2016 / RNAi-mediated Soybean mosaic virus (SMV) resistance of a Korean Soybean cultivar / Plant Biotechnology Reports 10(5):257~267
Hye Jeong Kim / 2013 / Characterization of SMV resistance of soybean produced by genetic transformation of SMV-CP gene in RNAi / Plant Biotechnology Reports 7(4):425~433
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