지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.10
- 수록면
- 425 - 433 (9page)
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초록· 키워드
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Soybean mosaic virus (SMV), a species of the Potyvirus genus in the Potyviridae family, is one of the most typical viral diseases and results in yield and quality loss of cultivated soybean. Due to the depletion of genetic resources for resistance breeding, a trial of genetic transformation to improve disease resistance has been performed by introducing the SMV-CP gene by the RNA interference (RNAi) method via Agrobacterium-mediated transformation. Among 30 transgenic plants produced, 7 lines with enough seeds were infected with SMV and two lines (3 and 4) showed viral resistance to SMV infection. In genomic Southern blot analysis, all the lines tested contained at least one T-DNA insertion. Subsequent investigation confirmed that no viral CP gene expression was detected in two SMV-resistant lines after artificial inoculation of SMV, while non-transgenic control and other transgenic lines expressed substantial amounts of the viral gene. Viral symptoms affected seed morphology, and clean seeds were harvested from the resistant lines. Also, strong viral gene expression was detected from the seeds of susceptible lines. In further generations, the same phenotypic appearance was maintained among non-transgenic and transgenic plants. Finally, the presence of helper component- proteinase (HC-Pro), known as a suppressor of gene silencing apparatus, was checked among transgenic lines. No expression of HC-Pro in resistant lines indicated that the viral CP-RNAi transformation into soybean somehow created a functional gene silencing system and resulted in a viral-resistant phenotype.
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참고문헌 (27)
참고문헌 신청
Anandalakshmi R. / 1998 / A viral suppressor of gene silencing in plants / Proc Natl Acad Sci USA 95:13079~13084
Atreya PL. / 1995 / Mutational analysis of the coat protein N-terminal amino acids involved in potyvirus transmission by aphids / J Gen Virol 76:265~270
Cho EK. / 1986 / Strain of soybean mosaic virus causing soybean necrotic disease in Korea / Korean J Breed 18(2):150~153
Di R. / 1996 / Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene / Plant Cell Rep 15:746~750
Gunduz I. / 2004 / Genetic and phenotypic analysis of soybean mosaic virus resistance in PI 88788 soybean / Phytopathology 94:687~692
Atreya PL. / 1995 / Mutational analysis of the coat protein N-terminal amino acids involved in potyvirus transmission by aphids / J Gen Virol 76:265~270
Cho EK. / 1986 / Strain of soybean mosaic virus causing soybean necrotic disease in Korea / Korean J Breed 18(2):150~153
Di R. / 1996 / Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene / Plant Cell Rep 15:746~750
Gunduz I. / 2004 / Genetic and phenotypic analysis of soybean mosaic virus resistance in PI 88788 soybean / Phytopathology 94:687~692
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