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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.8 No.1
- 발행연도
- 2019.1
- 수록면
- 27 - 34 (8page)
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Purpose: The aim of the present study was to develop a serodiagnostic test for differentiation infected from vaccinated animal (DIVA) strategy accompanying the marker vaccine lacking an immunodominant epitope (IDE) of nucleoprotein of Newcastle disease virus (NDV). Materials and Methods: Recombinant epitope-repeat protein (rERP) gene encoding eight repeats of the IDE sequence (ETQFLDLMRAVANSMR) by tetra-glycine linker was synthesized. Recombinant baculovirus carrying the rERP gene was generated to express the rERP in insect cells. Specificity and sensitivity of an indirect enzyme-linked immunosorbent assay (ELISA) employing the rERP was evaluated. Results: The rERP with molecular weight of 20 kDa was successfully expressed by the recombinant baculovirus in an insect-baculovirus system. The rERP was antigenically functional as demonstrated by Western blotting. An indirect ELISA employing the rERP was developed and its specificity and sensitivity was determined. The ELISA test allowed discrimination of NDV infected sera from epitope deletion virus vaccinated sera. Conclusion: The preliminary results represent rERP ELISA as a promising DIVA diagnostic tool.
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참고문헌 (29)
참고문헌 신청
Miller PJ / 2014 / Newcastle disease virus detection and differentiation from avian influenza / Methods Mol Biol 1161:235~239
Diel DG / 2012 / Genetic diversity of avian paramyxovirus type 1 : proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes / Infect Genet Evol 12:1770~1779
Mohan CM / 2006 / Recombinant haemagglutinin neuraminidase antigen-based single serum dilution ELISA for rapid serological profiling of Newcastle disease virus / J Virol Methods 138:117~122
Mebatsion T / 2002 / Newcastle disease virus(NDV)marker vaccine : an immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope / J Virol 76:10138~10146
Ahmad-Raus R / 2009 / Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies / Res Vet Sci 86:174~182
Diel DG / 2012 / Genetic diversity of avian paramyxovirus type 1 : proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes / Infect Genet Evol 12:1770~1779
Mohan CM / 2006 / Recombinant haemagglutinin neuraminidase antigen-based single serum dilution ELISA for rapid serological profiling of Newcastle disease virus / J Virol Methods 138:117~122
Mebatsion T / 2002 / Newcastle disease virus(NDV)marker vaccine : an immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope / J Virol 76:10138~10146
Ahmad-Raus R / 2009 / Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies / Res Vet Sci 86:174~182
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