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논문 기본 정보

자료유형
학술저널
저자정보
Ryan Nicholas (Medical Study Program, Faculty of Medicine, Universitas Tanjungpura, Pontianak, Indonesia) Sari Eka Pratiwi (Department of Biology and Pathobiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, Indonesia) Mardhia Mardhia (Department of Microbiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, Indonesia) Ysrafil Ysrafil (Department of Pharmacotherapy, Faculty of Medicine, Universitas Palangka Raya, Palangka Raya, Indonesia) Delima Fajar Liana (Department of Microbiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, Indonesia) Mahyarudin Mahyarudin (Department of Microbiology, Faculty of Medicine, Universitas Tanjungpura, Pontianak, Indonesia)
저널정보
질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Perspectives Vol.15 No.4
발행연도
2024.8
수록면
307 - 328 (22page)
DOI
10.24171/j.phrp.2024.0013

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Objectives: This study aimed to identify the optimal protein construction for designing a multi-epitope vaccine with both prophylactic and therapeutic effects against cervical cancer, utilizing an immunoinformatics approach. The construction process involved using capsid epitopes L1 and L2, as well as oncoproteins E5, E6, and E7 from human papillomavirus (HPV) types 16 and 18.Methods: An experimental in silico analysis with an immunoinformatics approach was used to develop 2 multi-epitope vaccine constructs (A and B). Further analysis was then conducted to compare the constructs and select the one with the highest potential against cervical cancer.Results: This study produced 2 antigenic, non-allergenic, and nontoxic multi-epitope vaccine constructs (A and B), which exhibited the ideal physicochemical properties for a vaccine. Further analysis revealed that construct B effectively induced both cellular and humoral immune responses.Conclusion: The multi-epitope vaccine construct B for HPV 16 and 18, designed for both prophylactic and therapeutic purposes, met the development criteria for a cervical cancer vaccine. However, these findings need to be validated through in vitro and in vivo experiments.

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