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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 405 - 413 (9page)
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BACKGROUND: Xanthine derivatives have been used to treat a variety of medical conditions including respiratory disease and neural degeneration. However, few studies have reported their effects on bone regeneration. Therefore, we investigated the effects of KPR-A148, a synthetic xanthine derivative on osteoblast differentiation in vitro and bone regeneration in vivo. METHODS: The cytotoxicity of KPR-A148 was evaluated using MC3T3-E1 cells by the 3-(4,5-dimethylthiazolyl-2)-2,5- diphenyltertrazolium bromide assay. The effects of KPR-A148 on osteoblast differentiation were examined by alkaline phosphatase staining, Alizarin red S staining, and real-time PCR of osteoblast differentiation marker genes. To investigate the effects of KPR-A148 on in vivo bone regeneration, a KPR-A148-containing collagen sponge was implanted into a mouse calvarial defect and KPR-A148 was injected twice, weekly. Bone regeneration was evaluated quantitatively by micro-CT and qualitatively by hematoxylin and eosin, as well as Masson’s Trichrome staining. RESULTS: KPR-A148 did not show toxicity in the MC3T3-E1 cells and promoted osteoblast differentiation in a concentration- dependent manner. 10 lM of KPR-A148 showed the most significant effect on alkaline phospatase staining and matrix mineralization. KPR-A148 increased the expression of osteoblast marker genes in both the early and late stages of differentiation. In addition, KPR-A148 significantly induced new bone formation in the calvarial defect model. CONCLUSION: These results demonstrate that KPR-A148 strongly induces osteoblast differentiation and new bone formation. Therefore, it could be used as a potential therapeutic agent for regenerating bone following its destruction by disease or trauma.
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참고문헌 (30)
참고문헌 신청
Saurabh Shivalkar / 2017 / Solid Freeform Techniques Application in Bone Tissue Engineering for Scaffold Fabrication / 조직공학과 재생의학 14(3):187~200
Shivaji Kashte / 2017 / Artificial Bone via Bone Tissue Engineering: Current Scenario and Challenges / 조직공학과 재생의학 14(1):1~14
Li Y / 2015 / Bone defect animal models for testing efficacy of bone substitute biomaterials / J Orthop Translat 3:95~104
Ho-Shui-Ling A / 2018 / Bone regeneration strategies : engineered scaffolds, bioactive molecules and stem cells current stage and future perspectives / Biomaterials 180:143~162
Chamieh F / 2016 / Accelerated craniofacial bone regeneration through dense collagen gel scaffolds seeded with dental pulp stem cells / Sci Rep 6:38814
Shivaji Kashte / 2017 / Artificial Bone via Bone Tissue Engineering: Current Scenario and Challenges / 조직공학과 재생의학 14(1):1~14
Li Y / 2015 / Bone defect animal models for testing efficacy of bone substitute biomaterials / J Orthop Translat 3:95~104
Ho-Shui-Ling A / 2018 / Bone regeneration strategies : engineered scaffolds, bioactive molecules and stem cells current stage and future perspectives / Biomaterials 180:143~162
Chamieh F / 2016 / Accelerated craniofacial bone regeneration through dense collagen gel scaffolds seeded with dental pulp stem cells / Sci Rep 6:38814
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