지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Intervertebral disc(IVD) mainly consists of Annulus fibrosus(AF) and Nucleus pulposus(NP), playing a role of distributing a mechanical load on vertebral body. IVD tissue engineering has been developed the methods to achieve anatomic morphology and restoration of biological function. The goal of present study is to identify the possibilities for creating a substitute of IVD the morphology and biological functions are the same as undamaged complete IVD. To fabricate the AF and NP combine biphasic IVD tissue, AF tissue scaffolds have been printed by 3D bio-printing system with natural biomaterials and NP tissues have been prepared by scaffold-free culture system. We evaluated whether the combined structure of 3D printed AF scaffold and scaffold-free NP tissue construct could support the architecture and cell functions as IVD tissue. 3D printed AF scaffolds were printed with 60 degree angle stripe patterned lamella structure(the inner-diameter is 5mm, outer-diameter is 10 mm and height is 3 mm). In the cytotoxicity test, the 3D printed AF scaffold showed good cell compatibility. The results of histological and immunohistochemical staining also showed the newly synthesized collagens and glycosaminoglycans, which are specific makers of AF tissue. And scaffold-free NP tissue actively synthesized glycosaminoglycans and type 2 collagen, which are the major components of NP tissue. When we combined two engineered tissues to realize the IVD, combined biphasic tissues showed a good integration between the two tissues. In conclusion, this study describes the fabrication of Engineered biphasic IVD tissue by using enable techniques of tissue engineering. This fabricated biphasic tissue would be used as a model system for the study of the native IVD tissue. In the future, it may have the potential to replace the damaged IVD in the future.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
J.P. Urban / 2003 / Degeneration of the intervertebral disc / Arthritis Res. Ther. 5(3):120~130
S. Chan / 2012 / Intervertebral disc regeneration or repair with biomaterials and stem cell therapy-feasible or fiction / Swiss. Med. Wkly. 142:w13598
D.M. O'Halloran / 2007 / Tissue-engineering approach to regenerating the intervertebral disc / Tissue Eng. 13(8):1927~1954
S. Li / 2008 / Zonal variations in cytoskeletal element organization, mRNA and protein expression in the intervertebral disc / J. Anat. 213(6):725~732
D. Pereira / 2013 / Hydrogels in acellular and cellular strategies for intervertebral disc regeneration / J. Tissue Eng. Regen. Med. 7(2):85~98
S. Chan / 2012 / Intervertebral disc regeneration or repair with biomaterials and stem cell therapy-feasible or fiction / Swiss. Med. Wkly. 142:w13598
D.M. O'Halloran / 2007 / Tissue-engineering approach to regenerating the intervertebral disc / Tissue Eng. 13(8):1927~1954
S. Li / 2008 / Zonal variations in cytoskeletal element organization, mRNA and protein expression in the intervertebral disc / J. Anat. 213(6):725~732
D. Pereira / 2013 / Hydrogels in acellular and cellular strategies for intervertebral disc regeneration / J. Tissue Eng. Regen. Med. 7(2):85~98
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