Copro-molecular diagnosis of the Toxoplasmatinae subfamily in dog and cat populations in northern Iran

Leila Izadi; Shahabeddin Sarvi; Seyed Abdollah Hosseini; Afsaneh Amouei; Mehdi Sharif; Mohammad Taghi Rahimi; Tooran Nayeri; Ahmad Daryani

추천

검색

자료유형: 학술저널

저자정보: Leila Izadi (Toxoplasmosis Research Center Mazandaran University of Medical Sciences) Shahabeddin Sarvi (Toxoplasmosis Research Center Mazandaran University of Medical Sciences Sari Iran) Seyed Abdollah Hosseini (Mazandaran University of Medical Sciences) Afsaneh Amouei (Mazandaran University of Medical Sciences) Mehdi Sharif (Mazandaran University of Medical Sciences) Mohammad Taghi Rahimi (Shahrood University of Medical Sciences) Tooran Nayeri (Mazandaran University of Medical Sciences) Ahmad Daryani (Mazandaran University of Medical Sciences)

저널정보: 한국역학회 Epidemiology and Health Epidemiology and Health Vol.42

발행연도: 2020.1

수록면: 1 - 7 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

OBJECTIVES: The oocysts of the Toxoplasmatinae subfamily (Neospora caninum, Hammondia hammondi and H. heydorni, and Besnoitia besnoiti) are morphologically similar to Toxoplasma gondii, and indistinguishable from each other. This study investigated the prevalence of the Toxoplasmatinae subfamily in dog and cat fecal samples using a nested polymerase chain reaction method. METHODS: Overall, 200 fecal samples from domestic dogs (n=120) and cats (n=80) were collected from 15 farms in northern Iran. The samples were homogenized in 2.5% potassium dichromate solution and subsequently concentrated with sucrose solution. DNA was extracted from samples using a genomic DNA kit. Specific primers and the 18S rDNA gene were used to screen and detect all Toxoplasmatinae oocysts. RESULTS: Overall, 2.5% (3 of 120) and 22.5% (18 of 80) of the fecal samples collected from dogs and cats were infected with Toxoplasmatinae. In dogs, 2 samples were positive for N. caninum and 1 sample was positive for T. gondii. In cats, all 18 positive samples belonged to T. gondii. No contamination with H. heydorni was observed in dog fecal samples or H. hammondi and B. besnoiti in cat fecal samples. A phylogenetic analysis revealed that the T. gondii (cat) and N. caninum (dog) found had similarities with parasites reported from other regions of the world. CONCLUSIONS: This is the first study to provide data on the epidemiology of Toxoplasmatinae oocysts in Iran. The findings suggest that public-health monitoring for the effective control of feces from cats and dogs and improved pet hygiene habits are needed.

#Toxoplasmatinae #Dogs #Cats #Fecal samples #Iran

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!