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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 633 - 641 (9page)
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Background Although overexpression of synuclein gamma (SNCG) has been reported in several cancers, few studies have been performed onSNCG in endometrial carcinomas. Objective This study aimed to investigate the role of SNCG in the progression of endometrial carcinoma. Methods The expression pattern and function ofSNCG gene were analyzed using the Gene Expression Omnibus (GEO) and Gene Set Enrichment Analysis (GSEA) datasets. Two vector types, containing either SNCG or negative control shRNAs, were used to evaluate cell proliferation, apoptosis, and metastasis using Cell Counting Kit 8, colony formation, flow cytometry, wound-healing, transwell, and invasion assays. The relative protein levels of N-cadherin, E-cadherin, vimentin, p-PI3K, PI3K, p-AKT, AKT, p-ERK, and ERK were determined by western bloting. Results Our results revealed thatSNCG mRNA expression and SNCG protein levels in shRNA-treated SPEC2 cells were lower than in the negative control cells. Furthermore, cell proliferation, migration, and invasion were significantly inhibited in SNCG shRNA-treated cells, but apoptosis was increased. The results of western blot analysis indicated that SNCG silencing reduced the protein levels of N-cadherin, vimentin, p-PI3K, p-AKT, and p-ERK, but not those of total PI3K, AKT, and ERK. Conclusions Therefore, shRNA-mediated suppression of SNCG inhibited SPEC2 cell proliferation, migration, and invasion, and promoted SPEC2 cell apoptosis, which was presumably accomplished via regulation of the PI3K/AKT/ERK signaling pathway.
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참고문헌 (36)
참고문헌 신청
Ahmad M / 2007 / Gamma-synuclein and the progression of cancer / FASEB J 21:3419~3430
Altomare DA / 2005 / Perturbations of the AKT signaling pathway in human cancer / Oncogene 24:7455~7464
Asati V / 2016 / PI3K/Akt/mTOR and Ras/Raf/MEK/ERK signaling pathways inhibitors as anticancer agents : structural and pharmacological perspectives / Eur J Med Chem 109:314~341
Ball HG / 1996 / Do we know the best therapy for early endometrial cancer? / Gynecol Oncol 60:173~175
Bruening W / 2000 / Synucleins are expressed in the majority of breast and ovarian carcinomas and in preneoplastic lesions of the ovary / Cancer 88:2154~2163
Altomare DA / 2005 / Perturbations of the AKT signaling pathway in human cancer / Oncogene 24:7455~7464
Asati V / 2016 / PI3K/Akt/mTOR and Ras/Raf/MEK/ERK signaling pathways inhibitors as anticancer agents : structural and pharmacological perspectives / Eur J Med Chem 109:314~341
Ball HG / 1996 / Do we know the best therapy for early endometrial cancer? / Gynecol Oncol 60:173~175
Bruening W / 2000 / Synucleins are expressed in the majority of breast and ovarian carcinomas and in preneoplastic lesions of the ovary / Cancer 88:2154~2163
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