지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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The microRNA (miR)-10b is the T helper (Th) 17 cell specific in patients with ankylosing spondylitis (AS). The interleukin (IL)-22, which is closely related to Th17 cells, has been implicated in the regulation of new bone formation in experimental models. Therefore, the aim of this study was to evaluate whether miR-10b affects bone formation via the IL-22 pathway in AS. Methods. Primary CD4+ T cells from AS were purified and transfected with miR-10b, anti-miR-10b, or scramble. Cell-surface markers and cytokine expression were analyzed by flow cytometry and enzyme-linked immunosorbent assay. Primary bone-derived cells (BdCs) from the facet joints of the spine were isolated, then osteogenic differentiation of primary BdCs was performed. We assessed alkaline phosphatase (ALP) activity and staining of BdCs at early time points. Alizarin red S staining of BdCs was performed at late time points. Results. Overexpression of miR-10b reduced both IL-22 producing cell frequencies and cytokine production in T cells from the patients with AS. The IL-22 significantly increased ALP staining and bone mineralization. The ALP promotor activity of AS-BdCs was notably higher for the IL-22 concentration. The supernatants of the miR-10b overexpression group suppressed ALP activity on osteogenic progenitor cells from the facet joints of the spine in patients with AS. Conclusion. Our data suggest that miR-10b suppresses IL-22 production, which was involved in osteogenic proliferation in AS. Therefore, miR-10b might be a potential therapeutic candidate for regulation of new bone formation in patients with AS.
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참고문헌 (29)
참고문헌 신청
Khan MA / 1990 / A wider spectrum of spondyloarthropathies / Semin Arthritis Rheum 20:107~113
Zheng Y / 2007 / Interleukin-22, a T(H)17 cytokine, mediates IL-23-induced dermal inflammation and acanthosis / Nature 445:648~651
Bowness P / 2011 / Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis / J Immunol 186:2672~2680
Jandus C / 2008 / Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides / Arthritis Rheum 58:2307~2317
Xueyi L / 2013 / Levels of circulating Th17 cells and regulatory T cells in ankylosing spondylitis patients with an inadequate response to anti-TNF-α therapy / J Clin Immunol 33:151~161
Zheng Y / 2007 / Interleukin-22, a T(H)17 cytokine, mediates IL-23-induced dermal inflammation and acanthosis / Nature 445:648~651
Bowness P / 2011 / Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis / J Immunol 186:2672~2680
Jandus C / 2008 / Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides / Arthritis Rheum 58:2307~2317
Xueyi L / 2013 / Levels of circulating Th17 cells and regulatory T cells in ankylosing spondylitis patients with an inadequate response to anti-TNF-α therapy / J Clin Immunol 33:151~161
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