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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 734 - 742 (9page)
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Purpose: The present study aimed to identify the physiological characteristics of cells by investigating the change in gene expressionand protein levels during extracellular matrix (ECM) synthesis in the intervertebral disc (IVD) under hypoxic conditions. Materials and Methods: To test the effect of oxygen on cell growth and ECM synthesis of chondrocyte-like cells, the cells from IVDwere separated and cultured in two hypoxia-mimicking systems: chemical hypoxic conditions using deferoxamine (DFO), andphysiological hypoxic conditions using a hypoxic chamber for 7 days. Chondrocyte like cells cultured without DFO and under thenormal oxygen concentration (21% O2 and 5% CO2, 37°C) served as the controls. Results: Chondrocyte-like cells cultured in the presence of 6% oxygen demonstrated a 100% increase in cellular proliferation comparedto the control. The cells treated with chemical hypoxic conditions demonstrated a dose-dependent increase in the mRNAexpression of glucose transporter-1, GAPDH, aggrecan, and type II collagen on Day 1. When treated with 100 μM DFO, the cellsshowed a 50% increase in the levels of proteoglycan protein on Day 7. The cells treated with chemical hypoxic condition demonstratedincrease in sulfated glycosaminoglycan (GAG) protein levels on Day 7. Moreover, the cells cultured in the presence of 6%oxygen showed a 120% increase in sulfated GAG levels on Day 7. Conclusion: The oxygen concentration had an important role in the viability, proliferation, and maturation of chondrocyte-likecells in IVD. In addition, chondrocyte-like cells are sensitive to the concentration of oxygen.
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